Evidence map›Paper›PMID 27457123›Full record

ArticlePlant physiology2016

Integrative Analyses of Nontargeted Volatile Profiling and Transcriptome Data Provide Molecular Insight into VOC Diversity in Cucumber Plants (Cucumis sativus).

Guo Wei, Peng Tian, Fengxia Zhang, Hao Qin, Han Miao, Qingwen Chen, Zhongyi Hu, Li Cao, Meijiao Wang, Xingfang Gu and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed, 129 citations in OpenAlex.

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8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Guo WeiState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Peng TianState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Fengxia ZhangState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Hao QinState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Han MiaoState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Qingwen ChenState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Zhongyi HuState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Li CaoState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).ORCID 0000-0002-9830-0102
Meijiao WangState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Xingfang GuState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).ORCID 0000-0001-7773-5150
Sanwen HuangState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).ORCID 0000-0002-8547-5309
Mingsheng ChenState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.).
Guodong WangState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (G.We., P.T., F.Z., H.Q., Q.C., Z.H., L.C., M.W., M.C., G.Wa.);University of the Chinese Academy of Sciences, Beijing 100039, China (G.We., P.T., Q.C., Z.H.); andKey Laboratory of Horticultural Crops Genetic Improvement of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China (H.M., X.G., S.H.) gdwang@genetics.ac.cn.ORCID 0000-0001-9917-0656
Chinese Academy of Sciences · CNState Key Laboratory of Plant Genomics · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant volatile organic compounds, which are generated in a tissue-specific manner, play important ecological roles in the interactions between plants and their environments, including the well-known functions of attracting pollinators and protecting plants from herbivores/fungi attacks. However, to date, there have not been reports of holistic volatile profiling of the various tissues of a single plant species, even for the model plant species. In this study, we qualitatively and quantitatively analyzed 85 volatile chemicals, including 36 volatile terpenes, in 23 different tissues of cucumber (Cucumis sativus) plants using solid-phase microextraction combined with gas chromatography-mass spectrometry. Most volatile chemicals were found to occur in a highly tissue-specific manner. The consensus transcriptomes for each of the 23 cucumber tissues were generated with RNA sequencing data and used in volatile organic compound-gene correlation analysis to screen for candidate genes likely to be involved in cucumber volatile biosynthetic pathways. In vitro biochemical characterization of the candidate enzymes demonstrated that TERPENE SYNTHASE11 (TPS11)/TPS14, TPS01, and TPS15 were responsible for volatile terpenoid production in the roots, flowers, and fruit tissues of cucumber plants, respectively. A functional heteromeric geranyl(geranyl) pyrophosphate synthase, composed of an inactive small subunit (type I) and an active large subunit, was demonstrated to play a key role in monoterpene production in cucumber. In addition to establishing a standard workflow for the elucidation of plant volatile biosynthetic pathways, the knowledge generated from this study lays a solid foundation for future investigations of both the physiological functions of cucumber volatiles and aspects of cucumber flavor improvement.

Indexed as

Gene Expression Regulation, PlantAmino Acid SequenceCluster AnalysisCucumis sativusGas Chromatography-Mass SpectrometryGene Expression ProfilingGenes, PlantMetabolic Networks and PathwaysMolecular StructurePhylogenyPlant ProteinsPlant RootsProtein BindingReverse Transcriptase Polymerase Chain ReactionSequence Analysis, RNASequence Homology, Amino AcidPlant ProteinsVolatile Organic Compounds

Identifiers

PMID27457123
PMCPMC5074635
OpenAlexW2487183921

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.