ArticleThe Journal of cell biology1989
Progressive and spatially differentiated stability of microtubules in developing neuronal cells.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 168 citations in OpenAlex.
- Article
- Towards a systems-level view of the microtubule cytoskeleton and its functions in physiology and disease.Nature reviews. Molecular cell biology · 2026Review
- Role of Cytoskeletal Elements in Regulation of Synaptic Functions: Implications Toward Alzheimer's Disease and Phytochemicals-Based Interventions.Molecular neurobiology · 2024Review
- Bridging the gap of axonal regeneration in the central nervous system: A state of the art review on central axonal regeneration.Frontiers in neuroscience · 2022Review
- AExperimental neurobiology · 2018Article
- Moonlighting Motors: Kinesin, Dynein, and Cell Polarity.Trends in cell biology · 2017Review
- Stability properties of neuronal microtubules.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Microtubules in health and degenerative disease of the nervous system.Brain research bulletin · 2016Review
- A Case for Microtubule Vulnerability in Amyotrophic Lateral Sclerosis: Altered Dynamics During Disease.Frontiers in cellular neuroscience · 2016Review
- Endothelial Barrier and Its Abnormalities in Cardiovascular Disease.Frontiers in physiology · 2015Review
- In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.Developmental cell · 2014Article
- The emerging role of forces in axonal elongation.Progress in neurobiology · 2011Review
- A molecular and structural mechanism for G protein-mediated microtubule destabilization.The Journal of biological chemistry · 2011Article
- Swimming against the tide: mobility of the microtubule-associated protein tau in neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Direct evidence for coherent low velocity axonal transport of mitochondria.The Journal of cell biology · 2006Article
- Role of actin filaments in the axonal transport of microtubules.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Rapid microtubule-dependent induction of neurite-like extensions in NIH 3T3 fibroblasts by inhibition of ROCK and Cbl.Molecular biology of the cell · 2003Article
- Live-cell imaging of slow axonal transport in cultured neurons.Methods in cell biology · 2003Article
- Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.Molecular biology of the cell · 2001Article
- Different contributions of microtubule dynamics and transport to the growth of axons and collateral sprouts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The establishment of neural circuits requires both stable and plastic properties in the neuronal cytoskeleton. In this study we show that properties of stability and lability reside in microtubules and these are governed by cellular differentiation and intracellular location. After culture for 3, 7, and 14 d in nerve growth factor-containing medium, PC-12 cells were microinjected with X-rhodamine-labeled tubulin. 8-24 h later, cells were photobleached with a laser microbeam at the cell body, neurite shaft, and growth cone. Replacement of fluorescence in bleached zones was monitored by digital video microscopy. In 3-d cultures, fluorescence recovery in all regions occurred by 26 +/- 17 min. Similarly, in older cultures, complete fluorescence recovery at the cell body and growth cone occurred by 10-30 min. However, in neurite shafts, fluorescence recovery was markedly slower (71 +/- 48 min for 7-d and 201 +/- 94 min for 14-d cultures). This progressive increase in the stability of microtubules in the neurite shafts correlated with an increase of acetylated microtubules. Acetylated microtubules were present specifically in the neurite shaft and not in the regions of fast microtubule turnover, the cell body and growth cone. During the recovery of fluorescence, bleached zones did not move with respect to the cell body. We conclude that the microtubule component of the neuronal cytoskeleton is differentially dynamic but stationary.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.