ArticleNature biotechnology2016
Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies.
Article in Nature biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 427 papers.
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The trial behind it
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Who cites it
427 citing papers in PubMed.
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- Genital microbiota of women using a 90 day tenofovir or tenofovir and levonorgestrel intravaginal ring in a placebo controlled randomized safety trial in Kenya.Scientific reports · 2022Trial
- Adjustable Intragastric Balloon Leads to Significant Improvement in Obesity-Related Lipidome and Fecal Microbiome Profiles: A Proof-of-Concept Study.Clinical and translational gastroenterology · 2022Trial
- Lasting shift in the gut microbiota in patients with acute myeloid leukemia.Blood advances · 2022Trial
- Regionally Divergent Relationships Between Microbial Communities and Environmental Gradients in Potato Production Systems.Environmental microbiology reports · 2026Article
- Soilless farming system design impacts the diversity and composition of microbiota.Applied and environmental microbiology · 2026Article
- Increasing usable reads in RNA-seq protocols.iScience · 2026Article
- Identification of a conserved GNAT-family lysine acetyltransferase inJournal of bacteriology · 2026Article
- Single-cell detection and quantification of the microbiota by MicFLY.Cell host & microbe · 2026Article
- Benchmarking Full-Length ITS Metabarcoding Across Illumina 2 × 500, PacBio, and Oxford Nanopore Sequencing Using Mock and Soil Communities.Molecular ecology resources · 2026Article
- Differentiation of the Foliar Microbiomes From Co-Occurring Grass Species Reflects Their Recency of Invasion.Ecology and evolution · 2026Article
- Article
- Field-to-forest fungal spillover is a biotic edge effect of coffee in Costa Rica.Nature communications · 2026Article
- Multi-omics links microbial dysbiosis, systemic inflammation, and metabolomic disruptions to SNAE risk in treated HIV.JCI insight · 2026Article
- Age-Related Microbial Differences in Newly Diagnosed Crohn's Disease Reveal the Distinct Enrichment of Oral-Associated Taxa in Pediatric Patients.Gut and liver · 2026Article
- CD69 Regulates Gingival Inflammation and Microbiome in Periodontitis.Journal of dental research · 2026Article
- Saccharomycetes and Malassezia fungi associate with early-life gut maturation and allergic disease risk in childhood.Nature communications · 2026Article
- Enzymatic quorum quenching alters the phyllosphere microbiome and suppresses a bacterial-induced plant disease.Scientific reports · 2026Article
- Wavyleaf basketgrass (mSphere · 2026Article
367 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Amplicon-based marker gene surveys form the basis of most microbiome and other microbial community studies. Such PCR-based methods have multiple steps, each of which is susceptible to error and bias. Variance in results has also arisen through the use of multiple methods of next-generation sequencing (NGS) amplicon library preparation. Here we formally characterized errors and biases by comparing different methods of amplicon-based NGS library preparation. Using mock community standards, we analyzed the amplification process to reveal insights into sources of experimental error and bias in amplicon-based microbial community and microbiome experiments. We present a method that improves on the current best practices and enables the detection of taxonomic groups that often go undetected with existing methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.