ArticlePloS one2016
Identification of SNP and SSR Markers in Finger Millet Using Next Generation Sequencing Technologies.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 89 citations in OpenAlex.
- Mainstreaming orphan millets for advancing climate smart agriculture to secure nutrition and health.Frontiers in plant science · 2022Pooled it
- Role of Millets for Food Security Under Climate Change.Plant-environment interactions (Hoboken, N.J.) · 2026Review
- The genomics of t'ef and finger millet domestication and spread.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Harnessing the potential of millets for climate-resilient and sustainable agriculture.Frontiers in plant science · 2025Review
- Genomic resources, opportunities, and prospects for accelerated improvement of millets.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Review
- Finger millet: a hero in the making to combat food insecurity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Review
- Genomic and phenotypic characterization of finger millet indicates a complex diversification history.The plant genome · 2024Article
- DArTseq-based SNP markers reveal high genetic diversity among early generation fall armyworm tolerant maize inbred lines.PloS one · 2024Article
- The potentialities of omics resources for millet improvement.Functional & integrative genomics · 2023Review
- Review
- Novel GBS-Based SNP Markers for Finger Millet and Their Use in Genetic Diversity Analyses.Frontiers in genetics · 2022Article
- Genome-Wide Assessment of Population Structure and Genetic Diversity of the Global Finger Millet Germplasm Panel Conserved at the ICRISAT Genebank.Frontiers in plant science · 2021Article
- Healthy and Resilient Cereals and Pseudo-Cereals for Marginal Agriculture: Molecular Advances for Improving Nutrient Bioavailability.Frontiers in genetics · 2020Review
- The taxonomic significance of ddRADseq based microsatellite markers in the closely related species of Heracleum (Apiaceae).PloS one · 2020Article
- Barnyard Millet for Food and Nutritional Security: Current Status and Future Research Direction.Frontiers in genetics · 2020Review
- Review
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Finger millet is an important cereal crop in eastern Africa and southern India with excellent grain storage quality and unique ability to thrive in extreme environmental conditions. Since negligible attention has been paid to improving this crop to date, the current study used Next Generation Sequencing (NGS) technologies to develop both Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNP) markers. Genomic DNA from cultivated finger millet genotypes KNE755 and KNE796 was sequenced using both Roche 454 and Illumina technologies. Non-organelle sequencing reads were assembled into 207 Mbp representing approximately 13% of the finger millet genome. We identified 10,327 SSRs and 23,285 non-homeologous SNPs and tested 101 of each for polymorphism across a diverse set of wild and cultivated finger millet germplasm. For the 49 polymorphic SSRs, the mean polymorphism information content (PIC) was 0.42, ranging from 0.16 to 0.77. We also validated 92 SNP markers, 80 of which were polymorphic with a mean PIC of 0.29 across 30 wild and 59 cultivated accessions. Seventy-six of the 80 SNPs were polymorphic across 30 wild germplasm with a mean PIC of 0.30 while only 22 of the SNP markers showed polymorphism among the 59 cultivated accessions with an average PIC value of 0.15. Genetic diversity analysis using the polymorphic SNP markers revealed two major clusters; one of wild and another of cultivated accessions. Detailed STRUCTURE analysis confirmed this grouping pattern and further revealed 2 sub-populations within wild E. coracana subsp. africana. Both STRUCTURE and genetic diversity analysis assisted with the correct identification of the new germplasm collections. These polymorphic SSR and SNP markers are a significant addition to the existing 82 published SSRs, especially with regard to the previously reported low polymorphism levels in finger millet. Our results also reveal an unexploited finger millet genetic resource that can be included in the regional breeding programs in order to efficiently optimize productivity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.