Evidence map›Paper›PMID 27454285›Full record

ArticleNature methods2016

Real-time selective sequencing using nanopore technology.

Matthew Loose, Sunir Malla, Michael Stout

Abstract read
In one paragraph

Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 233 papers.

0numbers the graph read from it
0cells of the map it votes in
233citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

233 citing papers in PubMed.

  1. Nanopore Sequencing for Chikungunya Virus: Principles and Application.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
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  3. Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Nanopore event detection in a simple and adaptive way.bioRxiv : the preprint server for biology · 2026
    Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

173 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Matthew LooseSchool of Life Sciences, University of Nottingham, Nottingham, UK.ORCID 0000-0002-5264-0929
Sunir MallaSchool of Life Sciences, University of Nottingham, Nottingham, UK.
Michael StoutSchool of Life Sciences, University of Nottingham, Nottingham, UK.

Funding

Biotechnology and Biological Sciences Research Council BB/M012336/1Biotechnology and Biological Sciences Research Council BB/M020061/1
6 · The paper itself

Abstract

The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.

Indexed as

NanoporesBacteriophage lambdaElectronic Data ProcessingGene LibraryGenome, HumanHigh-Throughput Nucleotide SequencingHumansNucleic Acid Amplification TechniquesRepetitive Sequences, Nucleic AcidSequence Analysis, DNATime Factors

Identifiers

PMID27454285
PMCPMC5008457

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.