ArticlePloS one2016
Establishment and Characterization of a Tumor Stem Cell-Based Glioblastoma Invasion Model.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Review
- Understanding current experimental models of glioblastoma-brain microenvironment interactions.Journal of neuro-oncology · 2024Review
- Review
- Advances in Research of Adult Gliomas.International journal of molecular sciences · 2021Review
- Chemotherapy-Induced Degradation of Glycosylated Components of the Brain Extracellular Matrix Promotes Glioblastoma Relapse Development in an Animal Model.Frontiers in oncology · 2021Article
- Androgen Receptor, Although Not a Specific Marker For, Is a Novel Target to Suppress Glioma Stem Cells as a Therapeutic Strategy for Glioblastoma.Frontiers in oncology · 2021Article
- Engineered Hydrogels for Brain Tumor Culture and Therapy.Bio-design and manufacturing · 2020Article
- Practical Review on Preclinical HumanCancers · 2020Review
- High-resolution tomographic analysis of in vitro 3D glioblastoma tumor model under long-term drug treatment.Science advances · 2020Article
- Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells.Oncology letters · 2019Article
- A three-dimensional (3D) organotypic microfluidic model for glioma stem cells - Vascular interactions.Biomaterials · 2019Article
- Integrating in vitro experiments with in silico approaches for Glioblastoma invasion: the role of cell-to-cell adhesion heterogeneity.Scientific reports · 2018Article
- A 3D topographical model of parenchymal infiltration and perivascular invasion in glioblastoma.APL bioengineering · 2018Article
- Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia.CNS oncology · 2018Article
- Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture.Disease models & mechanisms · 2018Article
- Shift of microRNA profile upon glioma cell migration using patient-derived spheroids and serum-free conditions.Journal of neuro-oncology · 2017Article
- Expression and prognostic impact of matrix metalloproteinase-2 (MMP-2) in astrocytomas.PloS one · 2017Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsGlioblastoma is the most frequent and malignant brain tumor. Recurrence is inevitable and most likely connected to tumor invasion and presence of therapy resistant stem-like tumor cells. The aim was therefore to establish and characterize a three-dimensional in vivo-like in vitro model taking invasion and tumor stemness into account.
methodsGlioblastoma stem cell-like containing spheroid (GSS) cultures derived from three different patients were established and characterized. The spheroids were implanted in vitro into rat brain slice cultures grown in stem cell medium and in vivo into brains of immuno-compromised mice. Invasion was followed in the slice cultures by confocal time-lapse microscopy. Using immunohistochemistry, we compared tumor cell invasion as well as expression of proliferation and stem cell markers between the models.
resultsWe observed a pronounced invasion into brain slice cultures both by confocal time-lapse microscopy and immunohistochemistry. This invasion closely resembled the invasion in vivo. The Ki-67 proliferation indexes in spheroids implanted into brain slices were lower than in free-floating spheroids. The expression of stem cell markers varied between free-floating spheroids, spheroids implanted into brain slices and tumors in vivo.
conclusionThe established invasion model kept in stem cell medium closely mimics tumor cell invasion into the brain in vivo preserving also to some extent the expression of stem cell markers. The model is feasible and robust and we suggest the model as an in vivo-like model with a great potential in glioma studies and drug discovery.
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