ArticlePloS one2016
Cell Electrosensitization Exists Only in Certain Electroporation Buffers.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
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Who cites it
22 citing papers in PubMed.
- Gold Nanoparticles-Enhanced Gene Transfer Driven by MHz-Frequency Nanosecond Pulsed Electric Fields.Biomolecules · 2025Article
- Reassessing lidocaine as an electroporation sensitizer in vitro.Scientific reports · 2025Article
- 3D-cell phantom-experimental setup to assess thermal effects and cell viability of lung tumor cells after electroporation.Scientific reports · 2024Article
- In Vitro Imaging and Molecular Characterization of CaInternational journal of molecular sciences · 2023Article
- Finite Element Evaluation of the Electric Field Distribution in a Non-Homogeneous Environment.Bioengineering (Basel, Switzerland) · 2023Article
- Basic Principles of RNA Interference: Nucleic Acid Types and In Vitro Intracellular Delivery Methods.Micromachines · 2023Review
- Scalable continuous-flow electroporation platform enabling T cell transfection for cellular therapy manufacturing.Scientific reports · 2023Article
- High-frequency irreversible electroporation brain tumor ablation: exploring the dynamics of cell death and recovery.Bioelectrochemistry (Amsterdam, Netherlands) · 2022Article
- Does the shape of the electric pulse matter in electroporation?Frontiers in oncology · 2022Review
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
- Understanding the role of calcium-mediated cell death in high-frequency irreversible electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2020Article
- Genetic Transformation of the Filamentous FungusFrontiers in microbiology · 2019Article
- Chemical Enhancement of Irreversible Electroporation: A Review and Future Suggestions.Technology in cancer research & treatment · 2019Review
- Characterization of Ablation Thresholds for 3D-Cultured Patient-Derived Glioma Stem Cells in Response to High-Frequency Irreversible Electroporation.Research (Washington, D.C.) · 2019Article
- Perspective on Pulsed Electric Field Treatment in the Bio-based Industry.Frontiers in bioengineering and biotechnology · 2019Article
- Electropermeabilization of cells by closely spaced paired nanosecond-range pulses.Bioelectrochemistry (Amsterdam, Netherlands) · 2018Article
- Membrane Permeabilization of Pathogenic Yeast in Alternating Sub-microsecond Electromagnetic Fields in Combination with Conventional Electroporation.The Journal of membrane biology · 2018Article
- Control by Low Levels of Calcium of Mammalian Cell Membrane Electropermeabilization.The Journal of membrane biology · 2018Article
- Physical and Chemical Enhancement of and Adaptive Resistance to Irreversible Electroporation of Pancreatic Cancer.Annals of biomedical engineering · 2018Article
- Characterization of Cell Membrane Permeability In Vitro Part II: Computational Model of Electroporation-Mediated Membrane Transport.Technology in cancer research & treatment · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment at the same cumulative energy input. It could be exploited in electroporation-based treatments such as electrochemotherapy and tissue ablation with irreversible electroporation. The mechanisms responsible for cell sensitization, however, have not yet been identified. We investigated cell sensitization dynamics in five different electroporation buffers. We split a pulse train into two trains varying the delay between them and measured the propidium uptake by fluorescence microscopy. By fitting the first-order model to the experimental results, we determined the uptake due to each train (i.e. the first and the second) and the corresponding resealing constant. Cell sensitization was observed in the growth medium but not in other tested buffers. The effect of pulse repetition frequency, cell size change, cytoskeleton disruption and calcium influx do not adequately explain cell sensitization. Based on our results, we can conclude that cell sensitization is a sum of several processes and is buffer dependent. Further research is needed to determine its generality and to identify underlying mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.