Evidence map›Paper›PMID 27454154›Full record

ArticleJournal of the American Chemical Society2016

Glyco-seek: Ultrasensitive Detection of Protein-Specific Glycosylation by Proximity Ligation Polymerase Chain Reaction.

Peter V Robinson, Cheng-Ting Tsai, Amber E de Groot, Julia L McKechnie, Carolyn R Bertozzi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Ultrasensitive, multiplexed chemoproteomic profiling with soluble activity-dependent proximity ligation.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Co-opting a Bioorthogonal Reaction for Oncometabolite Detection.Journal of the American Chemical Society · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Peter V RobinsonDepartments of †Chemistry, ‡Molecular and Cell Biology, and §Molecular Environmental Biology, University of California , Berkeley, California 94720, United States.
Cheng-Ting TsaiDepartments of †Chemistry, ‡Molecular and Cell Biology, and §Molecular Environmental Biology, University of California , Berkeley, California 94720, United States.
Amber E de GrootDepartments of †Chemistry, ‡Molecular and Cell Biology, and §Molecular Environmental Biology, University of California , Berkeley, California 94720, United States.
Julia L McKechnieDepartments of †Chemistry, ‡Molecular and Cell Biology, and §Molecular Environmental Biology, University of California , Berkeley, California 94720, United States.
Carolyn R BertozziDepartments of †Chemistry, ‡Molecular and Cell Biology, and §Molecular Environmental Biology, University of California , Berkeley, California 94720, United States.

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Revolutionizing the detection of O-GlcNAcR21DK108781 · NIDDK · STANFORD UNIVERSITY · PI BERTOZZI, CAROLYN · 2015 to 2016
$610k
Howard Hughes Medical InstituteNIDDK NIH HHS R21 DK108781NIGMS NIH HHS T32 GM066698
6 · The paper itself

Abstract

We report a non-destructive biochemical technique, termed "Glyco-seek", for analysis of O-GlcNAcylated proteins. Glyco-seek combines chemoenzymatic labeling, proximity ligation, and quantitative polymerase chain reaction to detect O-GlcNAcylated proteins with ultrahigh sensitivity. Our glycan-specific assay can be paired with traditional proximity ligation assays to simultaneously determine the change in total protein levels. We show that Glyco-seek detects attomoles of glycoproteins of interest from cell lysates, with sensitivity several orders of magnitude higher than that of current techniques. We used the method to directly assay the O-GlcNAcylation status of a low-abundance transcription factor from cell lysates without need for isolation or enrichment.

Indexed as

Limit of DetectionAcetylglucosamineAzidesClick ChemistryGlycoproteinsGlycosylationPolymerase Chain ReactionAcetylglucosamineAzidesGlycoproteins

Identifiers

PMID27454154
PMCPMC5327792

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.