ArticleBone reports2016
Loss of Iroquois homeobox transcription factors 3 and 5 in osteoblasts disrupts cranial mineralization.
Article in Bone reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- IRX3 controls a SUMOylation-dependent differentiation switch in adipocyte precursor cells.Nature communications · 2025Article
- Article
- GRAMMAR-Lambda Delivers Efficient Understanding of the Genetic Basis for Head Size in Catfish.Biology · 2025Article
- Irx3/5 Null Deletion in Mice Blocks Cochlea-Saccule Segregation and Disrupts the Auditory Tonotopic Map.The Journal of comparative neurology · 2024Article
- Article
- Decreased CRISPLD2 expression impairs osteogenic differentiation of human mesenchymal stem cells during in vitro expansion.Journal of cellular physiology · 2023Article
- IRX5 promotes DNA damage repair and activation of hair follicle stem cells.Stem cell reports · 2023Article
- Technologies, strategies, and cautions when deconvoluting genome-wide association signals: FTO in focus.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2023Review
- Deficiency of Irx5 protects mice from obesity and associated metabolic abnormalities.International journal of obesity (2005) · 2022Article
- IRX5 promotes adipogenesis of hMSCs by repressing glycolysis.Cell death discovery · 2022Article
- Clinical and Molecular Update on the Fourth Reported Family with Hamamy Syndrome.Molecular syndromology · 2021Article
- A comprehensive series of Irx cluster mutants reveals diverse roles in facial cartilage development.Development (Cambridge, England) · 2021Article
- Understanding the role of Iroquois homeobox transcription factor 5 (IRX5) in cardiac function: getting to the (human) heart of the matter.Cardiovascular research · 2021Article
- Regulation and Role of Transcription Factors in Osteogenesis.International journal of molecular sciences · 2021Review
- Irx3 and Irx5 in Ins2-CreNature metabolism · 2021Article
- hReg-CNCC reconstructs a regulatory network in human cranial neural crest cells and annotates variants in a developmental context.Communications biology · 2021Article
- Review
- Identification of Novel Genetic Variants Related to Trabecular Bone Score in Community-Dwelling Older Adults.Endocrinology and metabolism (Seoul, Korea) · 2020Article
- Bone morphology is regulated modularly by global and regional genetic programs.Development (Cambridge, England) · 2019Article
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Authors and funding
12 authors at 6 institutions in 5 countries.
Funding
Abstract
Cranial malformations are a significant cause of perinatal morbidity and mortality. Iroquois homeobox transcription factors (IRX) are expressed early in bone tissue formation and facilitate patterning and mineralization of the skeleton. Mice lacking Irx5 appear grossly normal, suggesting that redundancy within the Iroquois family. However, global loss of both Irx3 and Irx5 in mice leads to significant skeletal malformations and embryonic lethality from cardiac defects. Here, we study the bone-specific functions of Irx3 and Irx5 using Osx-Cre to drive osteoblast lineage-specific deletion of Irx3 in Irx5(-/-) mice. Although we found that the Osx-Cre transgene alone could also affect craniofacial mineralization, newborn Irx3 (flox/flox) /Irx5(-/-)/Osx-Cre (+) mice displayed additional mineralization defects in parietal, interparietal, and frontal bones with enlarged sutures and reduced calvarial expression of osteogenic genes. Newborn endochondral long bones were largely unaffected, but we observed marked reductions in 3-4-week old bone mineral content of Irx3 (flox/flox) /Irx5(-/-)/Osx-Cre (+) mice. Our findings indicate that IRX3 and IRX5 can work together to regulate mineralization of specific cranial bones. Our results also provide insight into the causes of the skeletal changes and mineralization defects seen in Hamamy syndrome patients carrying mutations in IRX5.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.