ArticleThe Journal of biological chemistry2016
Key Residues for Catalytic Function and Metal Coordination in a Carotenoid Cleavage Dioxygenase.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 30 citations in OpenAlex.
- Mechanism of Lutein tobioRxiv : the preprint server for biology · 2025Article
- Spectroscopy and crystallography define carotenoid oxygenases as a new subclass of mononuclear non-heme FeThe Journal of biological chemistry · 2025Article
- Article
- Carotenoid Cleavage Dioxygenase GeneInternational journal of molecular sciences · 2024Article
- Characteristics of a new carotenoid cleavage dioxygenase NtCCD10 derived from Nicotiana tabacum.Planta · 2022Article
- Retinal pigment epithelium 65 kDa protein (RPE65): An update.Progress in retinal and eye research · 2022Review
- Preparation of carotenoid cleavage dioxygenases for X-ray crystallography.Methods in enzymology · 2022Article
- GmCCD4 controls carotenoid content in soybeans.Plant biotechnology journal · 2021Article
- Molecular components affecting ocular carotenoid and retinoid homeostasis.Progress in retinal and eye research · 2021Review
- Assessing the Role ofFrontiers in nutrition · 2021Article
- Structural and mechanistic aspects of carotenoid cleavage dioxygenases (CCDs).Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Structural basis for carotenoid cleavage by an archaeal carotenoid dioxygenase.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Crocetin Overproduction in EngineeredFrontiers in bioengineering and biotechnology · 2020Article
- Identification of functionally important residues and structural features in a bacterial lignostilbene dioxygenase.The Journal of biological chemistry · 2019Article
- Preparation and characterization of metal-substituted carotenoid cleavage oxygenases.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- Resveratrol as a Growth Substrate for Bacteria from the Rhizosphere.Applied and environmental microbiology · 2018Article
- Structural and functional analysis of lignostilbene dioxygenases from Sphingobium sp. SYK-6.The Journal of biological chemistryArticle
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5 authors at 1 institution in 1 country.
Funding
Abstract
Carotenoid cleavage dioxygenases (CCDs) are non-heme iron-containing enzymes found in all domains of life that generate biologically important apocarotenoids. Prior studies have revealed a critical role for a conserved 4-His motif in forming the CCD iron center. By contrast, the roles of other active site residues in catalytic function, including maintenance of the stringent regio- and stereo-selective cleavage activity, typically exhibited by these enzymes have not been thoroughly investigated. Here, we examined the functional and structural importance of active site residues in an apocarotenoid-cleaving oxygenase (ACO) from Synechocystis Most active site substitutions variably lowered maximal catalytic activity without markedly affecting the Km value for the all-trans-8'-apocarotenol substrate. Native C15-C15' cleavage activity was retained in all ACO variants examined suggesting that multiple active site residues contribute to the enzyme's regioselectivity. Crystallographic analysis of a nearly inactive W149A-substituted ACO revealed marked disruption of the active site structure, including loss of iron coordination by His-238 apparently from an altered conformation of the conserved second sphere Glu-150 residue. Gln- and Asp-150-substituted versions of ACO further confirmed the structural/functional requirement for a Glu side chain at this position, which is homologous to Glu-148 in RPE65, a site in which substitution to Asp has been associated with loss of enzymatic function in Leber congenital amaurosis. The novel links shown here between ACO active site structure and catalytic activity could be broadly applicable to other CCD members and provide insights into the molecular pathogenesis of vision loss associated with an RPE65 point mutation.
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