ArticleCell systems2016
Deep Proteome Analysis Identifies Age-Related Processes in C. elegans.
Article in Cell systems, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed, 124 citations in OpenAlex.
- The Extended Lifespan ofCurrent issues in molecular biology · 2026Article
- Mitochondria-lysosome coupling contributes to lysosome acidification and aging.Molecular cell · 2026Article
- Peroxisomes orchestrate metabolic flexibility and longevity via an interorganelle cascade.Nature aging · 2026Article
- Review
- Aging impairs peroxisome biogenesis in human B cells.The journals of gerontology. Series A, Biological sciences and medical sciences · 2026Article
- Multilayered regulation of longevity in Caenorhabditis elegans.Molecules and cells · 2026Review
- A practical guide to sample preparation for immunoprecipitation coupled with mass spectrometry analysis ofmicroPublication biology · 2026Article
- Transcriptomic insights into the healthspan-enhancing effects of C. chinensis seed and E. ulmoides bark extracts in Caenorhabditis elegans.Biogerontology · 2025Article
- Alterations in peroxisome-mitochondria interplay in skeletal muscle accelerate muscle dysfunction.Nature communications · 2025Article
- Simple In-Cell Processing Enables Deep Proteome Analysis of Low-InputAnalytical chemistry · 2025Article
- PEX1The Journal of biological chemistry · 2025Article
- Mitochondrial Proteome Reveals Metabolic Tuning by Restricted Insulin Signaling to Promote Longevity inBiology · 2025Article
- Detecting gene expression in Caenorhabditis elegans.Genetics · 2025Review
- ELO-6 expression predicts longevity in isogenic populations of Caenorhabditis elegans.Nature communications · 2024Article
- Amyloid β accelerates age-related proteome-wide protein insolubility.GeroScience · 2024Article
- Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis.PNAS nexus · 2024Article
- Single-tissue proteomics inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Queen bee gut microbiota extends honeybee lifespan by inhibiting insulin signaling.Applied and environmental microbiology · 2024Article
- Enhanced branched-chain amino acid metabolism improves age-related reproduction in C. elegans.Nature metabolism · 2024Article
- Single-worm quantitative proteomics reveals aging heterogeneity in isogenic Caenorhabditis elegans.Aging cell · 2024Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Effective network analysis of protein data requires high-quality proteomic datasets. Here, we report a near doubling in coverage of the C. elegans adult proteome, identifying >11,000 proteins in total with ∼9,400 proteins reproducibly detected in three biological replicates. Using quantitative mass spectrometry, we identify proteins whose abundances vary with age, revealing a concerted downregulation of proteins involved in specific metabolic pathways and upregulation of cellular stress responses with advancing age. Among these are ∼30 peroxisomal proteins, including the PRX-5/PEX5 import protein. Functional experiments confirm that protein import into the peroxisome is compromised in vivo in old animals. We also studied the behavior of the set of age-variant proteins in chronologically age-matched, long-lived daf-2 insulin/IGF-1-pathway mutants. Unexpectedly, the levels of many of these age-variant proteins did not scale with extended lifespan. This indicates that, despite their youthful appearance and extended lifespans, not all aspects of aging are reset in these long-lived mutants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.