ArticleReproductive toxicology (Elmsford, N.Y.)2016
Optimizing multi-dimensional high throughput screening using zebrafish.
Article in Reproductive toxicology (Elmsford, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 69 citations in OpenAlex.
- Article
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- Position-specific methyl substitution on benzo[a]pyrene drives AHR-dependent fin duplication in zebrafish.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Aip mutation leads to significant alterations in gene expression, altered sensitivity to Ahr ligands, and early lethality in zebrafish.Frontiers in toxicology · 2026Article
- Adverse adult-onset and multigenerational effects in zebrafish (Danio rerio) developmentally exposed to polystyrene nanoplastics.Environmental pollution (Barking, Essex : 1987) · 2025Article
- Chemical structure drives developmental toxicity of alkyl-substituted naphthalenes in zebrafish.Environment international · 2025Article
- Article
- Integrating network analysis and machine learning to elucidate chemical-induced pancreatic toxicity in zebrafish embryos.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Zebrafish as a Model for Translational Immuno-Oncology.Journal of personalized medicine · 2025Review
- Advancements in the Developmental Zebrafish Model for Predictive Human Toxicology.Current opinion in toxicology · 2025Article
- Accelerating antiviral drug discovery: early hazard detection with a dual zebrafish and cell culture screen of a 403 compound library.Archives of toxicology · 2025Article
- Developmental toxicity of alkylated PAHs and substituted phenanthrenes: Structural nuances drive diverse toxicity and AHR activation.Chemosphere · 2025Article
- Rapid determination of chemical losses in a microplate bioassay using fluorescence spectroscopy.Analytical methods : advancing methods and applications · 2025Article
- Deep autoencoder-based behavioral pattern recognition outperforms standard statistical methods in high-dimensional zebrafish studies.PLoS computational biology · 2024Article
- PAH bioremediation with Rhodococcus rhodochrous ATCC 21198: Impact of cell immobilization and surfactant use on PAH treatment and post-remediation toxicity.Journal of hazardous materials · 2024Article
- Article
- Comparison of Pronase versus Manual Dechorionation of Zebrafish Embryos for Small Molecule Treatments.Journal of developmental biology · 2023Article
- Systematic developmental toxicity assessment of a structurally diverse library of PFAS in zebrafish.Journal of hazardous materials · 2022Article
- An assessment of vaping-induced inflammation and toxicity: A feasibility study using a 2-stage zebrafish and mouse platform.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2022Article
- A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals.International journal of environmental research and public health · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The use of zebrafish for high throughput screening (HTS) for chemical bioactivity assessments is becoming routine in the fields of drug discovery and toxicology. Here we report current recommendations from our experiences in zebrafish HTS. We compared the effects of different high throughput chemical delivery methods on nominal water concentration, chemical sorption to multi-well polystyrene plates, transcription responses, and resulting whole animal responses. We demonstrate that digital dispensing consistently yields higher data quality and reproducibility compared to standard plastic tip-based liquid handling. Additionally, we illustrate the challenges in using this sensitive model for chemical assessment when test chemicals have trace impurities. Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.