ArticleScientific reports2016
A simple Gateway-assisted construction system of TALEN genes for plant genome editing.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- TALEN-Interceded Genome Editing in Plants: Unveiling New Frontiers in Secondary Metabolite Improvement and Genetic Diversity.Plants (Basel, Switzerland) · 2025Review
- Potato: from functional genomics to genetic improvement.Molecular horticulture · 2024Review
- CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato.Transgenic research · 2023Article
- Efficiency of potato genome editing: Targeted mutation on the genes involved in starch biosynthesis using the CRISPR/dMac3-Cas9 system.Plant biotechnology (Tokyo, Japan) · 2023Article
- Review
- Recent Advances in Molecular Improvement for Potato Tuber Traits.International journal of molecular sciences · 2022Review
- A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises.Current issues in molecular biology · 2021Review
- Creation of a potato mutant lacking the starch branching enzyme genePlant biotechnology (Tokyo, Japan) · 2021Article
- State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential.Frontiers in plant science · 2021Review
- A simple method to establish an efficient medium suitable for potato regeneration.Plant biotechnology (Tokyo, Japan) · 2020Article
- Efficient genome engineering using Platinum TALEN in potato.Plant biotechnology (Tokyo, Japan) · 2019Article
- Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato.Scientific reports · 2018Article
- Applications of New Breeding Technologies for Potato Improvement.Frontiers in plant science · 2018Review
- Article
- Genome Editing for Crop Improvement - Applications in Clonally Propagated Polyploids With a Focus on Potato (Frontiers in plant science · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TALEN is an artificial nuclease being applied for sequence-specific genome editing. For the plant genome editing, a pair of TALEN genes is expressed in the cells, and a binary plasmid for Agrobacterium-mediated transformation should be assembled. We developed a novel procedure using the Gateway-assisted plasmids, named Emerald-Gateway TALEN system. We constructed entry vectors, pPlat plasmids, for construction of a desired TALEN gene using Platinum Gate TALEN kit. We also created destination plasmid, pDual35SGw1301, which allowed two TALEN genes to both DNA strands to recruit using Gateway technology. Resultant TALEN genes were evaluated by the single-strand annealing (SSA) assay in E. coli cells. By this assay, the TALENs recognized the corresponding targets in the divided luciferase gene, and induced a specific recombination to generate an active luciferase gene. Using the TALEN genes constructed, we created a transformant potato cells in which a site-specific mutation occurred at the target site of the GBSS gene. This suggested that our system worked effectively and was applicable as a convenient tool for the plant genome editing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.