ArticleCell reports2016
mRNA Cap Methylation in Pluripotency and Differentiation.
Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed.
- METTL1/WDR4 Complex: A Novel Epitranscriptomic Target at the Crossroads of RNA Methylation and Drug Discovery.Journal of medicinal chemistry · 2026Review
- Defining substrate specificities of human RNA capping methyltransferases through quantitative assessment of independent yet cooperative activities.Protein science : a publication of the Protein Society · 2026Article
- RNMT-dependent RNA cap methylation in health and disease.The Biochemical journal · 2025Review
- Characterisation of RNA guanine-7 methyltransferase (RNMT) using a small molecule approach.The Biochemical journal · 2025Article
- eIF4E orchestrates mRNA processing, RNA export and translation to modify specific protein production.Nucleus (Austin, Tex.) · 2024Review
- RAM is upregulated during T cell activation and is required for RNA cap formation and gene expression.Discovery immunology · 2024Article
- The RNA cap methyltransferases RNMT and CMTR1 co-ordinate gene expression during neural differentiation.Biochemical Society transactions · 2023Review
- A Paternal Methylation Error in the Congenital Hydrocephalic Texas (H-Tx) Rat Is Partially Rescued with Natural Folate Supplements.International journal of molecular sciences · 2023Article
- Cancer cells hijack RNA processing to rewrite the message.Biochemical Society transactions · 2022Article
- Review
- CMTR1 is recruited to transcription start sites and promotes ribosomal protein and histone gene expression in embryonic stem cells.Nucleic acids research · 2022Article
- Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.Journal of molecular biology · 2022Article
- Review
- Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation.Nucleic acids research · 2021Article
- To cap it all off, again: dynamic capping and recapping of coding and non-coding RNAs to control transcript fate and biological activity.Cell cycle (Georgetown, Tex.) · 2021Review
- CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures.Open biology · 2020Article
- Mechanism of allosteric activation of human mRNA cap methyltransferase (RNMT) by RAM: insights from accelerated molecular dynamics simulations.Nucleic acids research · 2019Article
- Oncogenic PIK3CA mutations increase dependency on the mRNA cap methyltransferase, RNMT, in breast cancer cells.Open biology · 2019Article
- mRNA cap regulation in mammalian cell function and fate.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
- DHX15 regulates CMTR1-dependent gene expression and cell proliferation.Life science alliance · 2018Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The mRNA cap recruits factors essential for transcript processing and translation initiation. We report that regulated mRNA cap methylation is a feature of embryonic stem cell (ESC) differentiation. Expression of the mRNA cap methyltransferase activating subunit RAM is elevated in ESCs, resulting in high levels of mRNA cap methylation and expression of a cohort of pluripotency-associated genes. During neural differentiation, RAM is suppressed, resulting in repression of pluripotency-associated factors and expression of a cohort of neural-associated genes. An established requirement of differentiation is increased ERK1/2 activity, which suppresses pluripotency-associated genes. During differentiation, ERK1/2 phosphorylates RAM serine-36, targeting it for ubiquitination and proteasomal degradation, ultimately resulting in changes in gene expression associated with loss of pluripotency. Elevated RAM expression also increases the efficiency of fibroblast reprogramming. Thus, the mRNA cap emerges as a dynamic mark that instructs change in gene expression profiles during differentiation and reprogramming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.