ArticlePlacenta2016
Human amnion mesenchymal cells are pro-inflammatory when activated by the Toll-like receptor 2/6 ligand, macrophage-activating lipoprotein-2.
Article in Placenta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Role of Myofibroblasts in the Repair of Iatrogenic Preterm Membranes Subjected to Mechanical Stimulation.Prenatal diagnosis · 2025Article
- Pushing the envelope: Immune mechanism and application landscape of macrophage-activating lipopeptide-2.Frontiers in immunology · 2023Review
- Fetal membrane at the feto-maternal interface: An underappreciated and understudied intrauterine tissue.Placenta and reproductive medicine · 2022Article
- Generation and characterization of human Fetal membrane and Decidual cell lines for reproductive biology experiments†.Biology of reproduction · 2022Article
- Fetal DNA Causes Sex-Specific Inflammation From Human Fetal Membranes.Frontiers in physiology · 2022Article
- Cx43 mediates changes in myofibroblast contraction and collagen release in human amniotic membrane defects after trauma.Scientific reports · 2021Article
- Transcriptional Profile of Cytokines, Regulatory Mediators and TLR in Mesenchymal Stromal Cells after Inflammatory Signaling and Cell-Passaging.International journal of molecular sciences · 2021Article
- Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth.Lab on a chip · 2021Article
- Progesterone receptor membrane components: key regulators of fetal membrane integrity.Biology of reproduction · 2021Article
- The Role of Innate Immune System in the Human Amniotic Membrane and Human Amniotic Fluid in Protection Against Intra-Amniotic Infections and Inflammation.Frontiers in immunology · 2021Review
- Modeling ascending infection with a feto-maternal interface organ-on-chip.Lab on a chip · 2020Article
- Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane Inflammation.International journal of molecular sciences · 2020Article
- Novel pathways of inflammation in human fetal membranes associated with preterm birth and preterm pre-labor rupture of the membranes.Seminars in immunopathology · 2020Review
- Reversible EMT and MET mediate amnion remodeling during pregnancy and labor.Science signaling · 2020Article
- The Role of Danger Associated Molecular Patterns in Human Fetal Membrane Weakening.Frontiers in physiology · 2020Review
- Organ-On-Chip Technology: The Future of Feto-Maternal Interface Research?Frontiers in physiology · 2020Review
- Fetal Membrane Epigenetics.Frontiers in physiology · 2020Review
- Oxidative stress-induced downregulation of glycogen synthase kinase 3 beta in fetal membranes promotes cellular senescence†.Biology of reproduction · 2019Article
- Distinct preterm labor phenotypes have unique inflammatory signatures and contraction associated protein profiles†.Biology of reproduction · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
introductionInfection accounts for over 40% of preterm premature rupture of the fetal membranes (PPROM), a major cause of preterm birth. Toll-like receptors (TLR) play key roles in pathogen surveillance but their expression and function in amnion mesenchymal cells (AMC) is unclear. The aims of this study were to determine the expression of all TLR isoforms and the effect of macrophage-activating lipoprotein-2 (MALP-2), derived from a common pathogen involved in PPROM, on human AMC.
methodsAMC were isolated from normal, term amnion from repeat cesarean section. Semi-quantitative RT-PCR, immunocytochemistry, immunohistochemistry and western blotting were used to detect TLR isoform expression. Immunocytochemistry of NF-κB p65, pro-inflammatory cytokine secretion (ELISA), MTT assay, LDH assay, immunoblotting of cytosolic cytochrome c and cleaved caspase-3, and expression of 84 microRNAs by Qiagen miRNA PCR array were used to determine the functional effect of MALP-2 on AMC.
resultsTLR1-10 was detected in AMC, and protein expression of TLR2, 4, and 6 were confirmed. MALP-2 induced nuclear translocation of p65, reaching significance after 45 min (ANOVA, P < 0.05). MALP-2 did not cause apoptosis but did lead to significant secretion of IL-4, IL-6, and IL-8 (P < 0.05, 0.01, 0.001, respectively) and significant changes in miRNA-320a and miRNA-18a (P < 0.05). DISCUSSION: These results suggest that AMC elicit a pro-inflammatory response following stimulation with the known TLR2/6 ligand MALP-2. This data supports the idea that AMC express the innate immune system receptors that could help with immune surveillance during infection and contribute to inflammatory responses that lead to PPROM.
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Registered trials
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