ArticleCytotechnology2016
Cellular conservation of endangered midget buffalo (Lowland Anoa, Bubalus quarlesi) by establishment of primary cultured cell, and its immortalization with expression of cell cycle regulators.
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Immortalization of primary cells derived from the endangered Ryukyu long-furred rat.In vitro cellular & developmental biology. Animal · 2023Article
- Immortalization of cells derived from domestic dogs through expressing mutant cyclin-dependent kinase 4, cyclin D1, and telomerase reverse transcriptase.Cytotechnology · 2022Article
- Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters.Cytotechnology · 2021Article
- The transcriptome of wild-type and immortalized corneal epithelial cells.Scientific data · 2021Article
- Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor.Frontiers in cell and developmental biology · 2020Article
- Article
- Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon).PloS one · 2019Article
- Human-Derived Corneal Epithelial Cells Expressing Cell Cycle Regulators as a New Resource forFrontiers in genetics · 2019Article
- Article
- Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta).Scientific reports · 2018Article
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lowland Anoa has become endangered due to hunting and human activity. Protection and breeding of endangered species in a controlled environment is the best way of conservation. However, it is not possible to adopt this approach for all endangered species because of the cost involved and the ever-increasing number of critically endangered species. In consideration of these limitations to the conventional conservation methods, we established a primary cell culture of endangered buffalo (Lowland Anoa, Bubalus quarlesi), for the preservation of this biological resource. In addition, we introduced human derived, mutant cyclin dependent kinase 4 (CDK4), Cyclin D, and telomerase reverse transcriptase (TERT) into the primary cells. The successful introduction of these three genes was confirmed by western blot with specific antibodies, and enzymatic activity. We also showed that the expression of mutant CDK4, Cyclin D, and TERT allows us to efficiently establish an immortalized cell line, with an intact chromosome pattern, from Lowland Anoa. To the best of our knowledge, this study is the first investigation that established an immortalized cell line of an endangered wild animal species.
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