ArticleRNA biology2016
Dual-function sRNA encoded peptide SR1P modulates moonlighting activity of B. subtilis GapA.
Article in RNA biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- YhfH functions as a tri-function RNA in Bacillus thuringiensis BMB171.Nucleic acids research · 2026Article
- Overview of small proteins encoded by bacterial dual-function small RNAs.Non-coding RNA research · 2025Review
- Review
- Disruption of the bacterial OLE RNP complex impairs growth on alternative carbon sources.PNAS nexus · 2024Article
- In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Small proteins in Gram-positive bacteria.FEMS microbiology reviews · 2023Article
- Article
- An RNA-binding protein acts as a major post-transcriptional modulator in Bacillus anthracis.Nature communications · 2022Article
- A dual-function RNA balances carbon uptake and central metabolism in Vibrio cholerae.The EMBO journal · 2021Article
- A new role for SR1 from Bacillus subtilis: regulation of sporulation by inhibition of kinA translation.Nucleic acids research · 2021Article
- β-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck.Nucleic acids research · 2021Review
- Understanding small ORF diversity through a comprehensive transcription feature classification.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Review
- Review
- Moonlighting inMicroorganisms · 2021Review
- SR7 - a dual-function antisense RNA fromRNA biology · 2021Article
- sRNA EsrE Is Transcriptionally Regulated by the Ferric Uptake Regulator Fur inJournal of microbiology and biotechnology · 2020Article
- Intermolecular Communication inFrontiers in molecular biosciences · 2020Review
- Bacterial RNA Degradosomes: Molecular Machines under Tight Control.Trends in biochemical sciences · 2020Review
- A new role for CsrA: promotion of complex formation between an sRNA and its mRNA target inRNA biology · 2019Article
- Dual-Function RNAs.Microbiology spectrum · 2018Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SR1 is a dual-function sRNA from B. subtilis that acts as a base-pairing regulatory RNA and as a peptide-encoding mRNA. Both functions of SR1 are highly conserved. Previously, we uncovered that the SR1 encoded peptide SR1P binds the glycolytic enzyme GapA resulting in stabilization of gapA mRNA. Here, we demonstrate that GapA interacts with RNases Y and J1, and this interaction was RNA-independent. About 1% of GapA molecules purified from B. subtilis carry RNase J1 and about 2% RNase Y. In contrast to the GapA/RNase Y interaction, the GapA/RNaseJ1 interaction was stronger in the presence of SR1P. GapA/SR1P-J1/Y displayed in vitro RNase activity on known RNase J1 substrates. Moreover, the RNase J1 substrate SR5 has altered half-lives in a ΔgapA strain and a Δsr1 strain, suggesting in vivo functions of the GapA/SR1P/J1 interaction. Our results demonstrate that the metabolic enzyme GapA moonlights in recruiting RNases while GapA bound SR1P promotes binding of RNase J1 and enhances its activity.
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