ArticleeLife2016
Mechanistic signs of double-barreled structure in a fluoride ion channel.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Asymmetry-induced distinct mechanisms and the transporting role of sodium in bacterial fluoride channel Fluc.bioRxiv : the preprint server for biology · 2026Article
- The molecular mechanism of fluoride export by the eukaryotic fluoride channel FEX.Nature communications · 2025Article
- Article
- Fluoride permeation mechanism of the Fluc channel in liposomes revealed by solid-state NMR.Science advances · 2023Article
- A molecular perspective on identifying TRPV1 thermosensitive regions and disentangling polymodal activation.Temperature (Austin, Tex.) · 2023Review
- Role of the CrcB transporter of Pseudomonas putida in the multi-level stress response elicited by mineral fluoride.Environmental microbiology · 2022Article
- Ion permeation, selectivity, and electronic polarization in fluoride channels.Biophysical journal · 2022Article
- Biocatalytic synthesis of 2-fluoro-3-hydroxypropionic acid.Frontiers in bioengineering and biotechnology · 2022Article
- An outer-pore gate modulates the pharmacology of the TMEM16A channel.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Membrane Exporters of Fluoride Ion.Annual review of biochemistry · 2021Review
- The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture.Methods in enzymology · 2021Article
- Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel.Science advances · 2020Article
- Mapping of Ion and Substrate Binding Sites in Human Sodium Iodide Symporter (hNIS).Journal of chemical information and modeling · 2020Article
- An Interfacial Sodium Ion is an Essential Structural Feature of Fluc Family Fluoride Channels.Journal of molecular biology · 2020Article
- Nitrate and Phosphate Transporters Rescue Fluoride Toxicity in Yeast.Chemical research in toxicology · 2019Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Article
- A CLC-type FNature structural & molecular biology · 2018Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The Fluc family of F(-) ion channels protects prokaryotes and lower eukaryotes from the toxicity of environmental F(-). In bacteria, these channels are built as dual-topology dimers whereby the two subunits assemble in antiparallel transmembrane orientation. Recent crystal structures suggested that Fluc channels contain two separate ion-conduction pathways, each with two F(-) binding sites, but no functional correlates of this unusual architecture have been reported. Experiments here fill this gap by examining the consequences of mutating two conserved F(-)-coordinating phenylalanine residues. Substitution of each phenylalanine specifically extinguishes its associated F(-) binding site in crystal structures and concomitantly inhibits F(-) permeation. Functional analysis of concatemeric channels, which permit mutagenic manipulation of individual pores, show that each pore can be separately inactivated without blocking F(-) conduction through its symmetry-related twin. The results strongly support dual-pathway architecture of Fluc channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.