ArticleParasite (Paris, France)2016
Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding.
Article in Parasite (Paris, France), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- PCR detection ofFood and waterborne parasitology · 2026Article
- Serological and molecular investigation ofFood and waterborne parasitology · 2026Article
- Risk Factors Associated withMicroorganisms · 2024Article
- Serological and molecular survey ofFrontiers in veterinary science · 2024Article
- High prevalence and diversity of Toxoplasma gondii DNA in feral cat feces from coastal California.PLoS neglected tropical diseases · 2023Article
- Toxoplasma gondii in the faeces of wild felids from the Atlantic Forest, Brazil.Memorias do Instituto Oswaldo Cruz · 2022Article
- Prevalence of Feline Immunodeficiency Virus andVeterinary sciences · 2021Article
- Molecular Methods for the Detection ofMicroorganisms · 2021Review
- Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia.Parasite (Paris, France) · 2020Article
- New prevalence surveillance ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019Article
- Detection of Toxoplasma gondii in faeces of privately owned cats using two PCR assays targeting the B1 gene and the 529-bp repetitive element.Parasitology research · 2017Article
- Detection of Echinococcus multilocularis and other foodborne parasites in fox, cat and dog faeces collected in kitchen gardens in a highly endemic area for alveolar echinococcosis.Parasite (Paris, France) · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
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Abstract
Detection of Toxoplasma gondii DNA in cat feces is considered indicative of the presence of T. gondii oocysts. This study aims to demonstrate that the high sensitivity of qPCR can lead to T. gondii DNA detection in cat feces in the absence of oocysts. A cat immune to toxoplasmosis was fed with a mouse experimentally infected with T. gondii. Detection of DNA of this parasite was performed by qPCR on feces passed: (i) on the day the cat ingested the infected prey; (ii) during the three previous days; and (iii) during the three following days. The kinetics of qPCR results are clearly not linked to oocyst shedding and this result demonstrates that qPCR can detect T. gondii DNA related to bradyzoites from an infected prey, in the absence of oocysts. Caution is thus recommended when interpreting T. gondii qPCR results for samples of cat feces.
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