Evidence map›Paper›PMID 27448803›Full record

ArticleBMC urology2016

MMP9 overexpression is associated with good surgical outcome in children with UPJO: Preliminary results.

Sabrina Thalita Reis, Kátia R M Leite, Nayara Izabel Viana, Roberto Iglesias Lopes, Caio Martins Moura, Renato F Ivanovic, Marcos Machado, Francisco Tibor Denes, Amilcar Giron, William Carlos Nahas and 2 more

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In one paragraph

Article in BMC urology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Sabrina Thalita ReisUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil. sasareis@gmail.com.
Kátia R M LeiteUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Nayara Izabel VianaUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Roberto Iglesias LopesUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Caio Martins MouraUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Renato F IvanovicUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Marcos MachadoUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Francisco Tibor DenesUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Amilcar GironUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
William Carlos NahasUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Miguel SrougiUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Carlo C PasserottiUrology Department, Laboratory of Medical Investigation (LIM55), University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil.
Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundUreteropelvic junction obstruction (UPJO) diagnosed prenatally occurs in 1:150 - 1:1200 pregnancies. Although many studies investigating the molecular changes of this obstructed segment have been performed, the underlying mechanisms are still unclear. The role of extracellular matrix (ECM) components remains controversial, and the investigations in the field of ECM changes, might help the better understanding of the pathogenesis of this common condition. The aim of the present study was to investigate for the first time in the literature whether MMP9 and its specific inhibitors, TIMP1 and RECK, are expressed in a reproducible, specific pattern in UPJ.

methodsUPJO specimens were obtained from 16 children at the time of dismembered pyeloplasty due to intrinsic UPJ stenosis. Expression levels of the three genes (MMP9, TIMP1 and RECK) were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Then correlated the expression levels of the genes according to grade study population that was divided in 2 categories according to Society of Fetal Urology classification, grade 3 (moderate) and 4 (severe). For DTPA we subdivided the childrens in 2 groups, obstructive (T 1/2 more than 20 min) and partial obstructive (T 1/2 between 10 and 20 min) and success in a surgery was defined as decrease in T 1/2 to less than 20 min, absence of symptoms, improving renal function and decreasing dilatation on successive exams.

resultsMMP9 was underexpressed and TIMP1 and RECK were overexpressed in children with obstructive DTPA but the differences were not statistically significant. Overexpression of MMP9 was higher among patients with severe grade of UPJ compared to those with moderate grade. Surprisingly expression levels of MMP-9 was three times higher in children who were successfully treated by surgery (n = 10) (p = 0.072), so those who were followed for at least 1 year after surgery and remained with improvement in renal function and decreasing dilation on intravenous urogram and TIMP-1 was underexpressed in 100 % of this cases (p = 0.00).

conclusionsWe showed an increase in expression of MMP9 and a decrease in expression of TIMP1 in children who improving renal function and decreasing dilation after surgery. We believe that the higher expression of MMP9 in these cases can reflect an increase in degradation and remodeling process that could be used as a marker for surgical outcome.

Indexed as

Kidney PelvisAdolescentChildChild, PreschoolFemaleGene Expression RegulationGPI-Linked ProteinsHumansInfantMaleMatrix Metalloproteinase 9Tissue Inhibitor of Metalloproteinase-1Treatment OutcomeUreteral ObstructionGPI-Linked ProteinsMatrix Metalloproteinase 9RECK protein, humanTIMP1 protein, humanTissue Inhibitor of Metalloproteinase-1ChildrenMetaloproteinasesUPJO

Identifiers

PMID27448803
PMCPMC4958283
OpenAlexW2499526085

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.