Evidence map›Paper›PMID 27448409›Full record

ArticleBMC musculoskeletal disorders2016

Effect of platelet mediator concentrate (PMC) on Achilles tenocytes: an in vitro study.

Esra Arslan, Thomas Nellesen, Andreas Bayer, Andreas Prescher, Sebastian Lippross, Sven Nebelung, Holger Jahr, Christine Jaeger, Wolf Dietrich Huebner, Horst Fischer and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC musculoskeletal disorders, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Growth factors in the treatment of Achilles tendon injury.Frontiers in bioengineering and biotechnology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Esra ArslanDepartment of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, D-52074, Aachen, Germany.
Thomas NellesenDepartment of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, D-52074, Aachen, Germany.
Andreas BayerDepartment of Heart and Vascular Surgery, University Hospital of Schleswig-Holstein Campus Kiel, Kiel, Germany.
Andreas PrescherDepartment of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, Germany.
Sebastian LipprossDepartment of Trauma Surgery, University Medical Center Schleswig-Holstein Kiel Campus, Kiel, Germany.
Sven NebelungDepartment of Orthopaedic Surgery, University Hospital of RWTH Aachen University, Aachen, Germany.
Holger JahrDepartment of Orthopaedic Surgery, University Hospital of RWTH Aachen University, Aachen, Germany.
Christine JaegerCurasan AG, Kleinostheim, Germany.
Wolf Dietrich HuebnerCurasan AG, Kleinostheim, Germany.
Horst FischerDepartment of Dental Materials and Biomaterials Research, University Hospital of RWTH Aachen University, Aachen, Germany.
Marcus StoffelInstitute of General Mechanics, RWTH Aachen University, Aachen, Germany.
Mehdi ShakibaeiInstitute of Anatomy Ludwig- Maximilians University Munich, Munich, Germany.
Thomas PufeDepartment of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, D-52074, Aachen, Germany.
Mersedeh TohidnezhadDepartment of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, D-52074, Aachen, Germany. mtohidnezhad@ukaachen.de.
RWTH Aachen University · DELudwig-Maximilians-Universität München · DEUniversity of Lübeck · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAlthough there are many studies discussing the etiological and pathological factors leading to both, acute and chronic tendon injuries, the pathophysiology of tendon injuries is still not clearly understood. Although most lesions are uncomplicated, treatment is long and unsatisfactory due to the poor vascularity of tendon tissue. Platelet mediator concentrate (PMC) contains many growth factors derived from platelets, which can promote wound healing. In this study we investigate the effects of PMC on tenocyte proliferation and differentiation in order to provide an experimental basis for tissue regeneration strategies and to develop new treatment concepts.

methodsUsing enzyme linked immunosorbent assay (ELISA) we were able to quantify the several growth factors and cytokines found in PMC. Tenocytes were isolated both from human and from mouse Achilles tendons and stimulated with PMC. CyQuant® and Cell Titer Blue® assays were carried out to analyze tendon growth and viability at different concentrations of PMC. Real time RT-PCR was used to analyze tenocyte gene expression with or without PMC treatment. Immunohistochemistry was carried out to detect the tenocyte-specific antibody tenomodulin (TNMD) and scleraxis (SCX).

resultsWe were able to detect numerous mediators such as platelet derived growth factor BB (PDGF-BB), interleukin 6 (IL-6), vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF-α), transforming growth factor beta 1 (TGF-ß1), and bone morphogenetic proteins 2, 4 and 7 (BMP-4, BMP-2, BMP-7) in PMC. It was possible to show a positive effect of PMC on human tendon cell growth and viability in a dose-dependent manner. Furthermore, PMC treatment led to induction of gene expression of scleraxis (SCX), type I collagen A 1 (Col1A1) and TNMD by tenocytes.

conclusionsWe suggest that the use of autologous PMC may be a suitable addition to conventional tendon therapy that is capable of increasing and optimizing tendon healing and reducing the risk of recurrence.

Indexed as

Achilles TendonAdolescentAdultAngiogenesis Inducing AgentsAnimalsBasic Helix-Loop-Helix ProteinsBecaplerminBlood PlateletsBone Morphogenetic ProteinsCell DifferentiationCell ProliferationCells, CulturedCollagen Type ICollagen Type I, alpha 1 ChainEnzyme-Linked Immunosorbent AssayFemaleAngiogenesis Inducing AgentsBasic Helix-Loop-Helix ProteinsBecaplerminBone Morphogenetic ProteinsCollagen Type ICollagen Type I, alpha 1 ChainMembrane ProteinsProto-Oncogene Proteins c-sisSCX protein, humanTNMD protein, humanVascular Endothelial Growth Factor APlateletPlatelet mediator concentrateScleraxisTenocyteTenomodulin

Identifiers

PMID27448409
PMCPMC4957296
OpenAlexW2477998519

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.