Evidence map›Paper›PMID 27447893›Full record

ArticlePlant cell reports2016

Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR.

Cintia Jozefkowicz, Emilia Bottero, Cecilia Pascuan, Elba Pagano, Nicolás Daniel Ayub, Gabriela Soto

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Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Cintia JozefkowiczConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Emilia BotteroInstituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
Cecilia PascuanConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Elba PaganoInstituto de Genética Ewald A. Favret (CICVyA-INTA), De los Reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
Nicolás Daniel AyubConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Gabriela SotoConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. soto.gabrielacinthia@inta.gob.ar.ORCID http://orcid.org/0000-0002-3492-1268

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is partially due to the high times and costs needed for the production of vast numbers of transgenic alfalfa events as an empirical approach to bypass the random transgenic silencing and for the identification of an event with optimal transgene expression. In this focus article, we report the complete sequence of pPZP200BAR and the extremely high efficiency of this binary vector in alfalfa transformation, opening the way for rapid and inexpensive production of transgenic events for alfalfa improvement public programs.

Indexed as

Costs and Cost AnalysisGene LibrarySequence Analysis, DNAGenetic TechniquesGenetic VectorsMedicago sativaPlants, Genetically ModifiedPlasmidsTime FactorsTransformation, GeneticAlfalfaBinary vectorComplete sequencepPZP200BARTransformation

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.