Evidence map›Paper›PMID 27446685›Full record

ArticleBiomedical optics express2016

In vivo imaging of airway cilia and mucus clearance with micro-optical coherence tomography.

Kengyeh K Chu, Carolin Unglert, Tim N Ford, Dongyao Cui, Robert W Carruth, Kanwarpal Singh, Linbo Liu, Susan E Birket, George M Solomon, Steven M Rowe and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 70 citations in OpenAlex.

  1. Trial
  2. Article
  3. Optical Imaging of Cilia in the Head and Neck.Journal of clinical medicine · 2025
    Review
  4. Article
  5. Article
  6. Journal of biomedical optics · 2024
    Article
  7. Optica · 2023
    Article
  8. Article
  9. Article
  10. Article
  11. Culture and Imaging of Human Nasal Epithelial Organoids.Journal of visualized experiments : JoVE · 2021
    Article
  12. In vivo optical molecular imaging of inflammation and immunity.Journal of molecular medicine (Berlin, Germany) · 2021
    Review
  13. CFTR function and clinical response to modulators parallel nasal epithelial organoid swelling.American journal of physiology. Lung cellular and molecular physiology · 2021
    Article
  14. Article
  15. Article
  16. Experimental biology and medicine (Maywood, N.J.) · 2020
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Kengyeh K ChuWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA; Contributed equally as co-authors.
Carolin UnglertWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA; Contributed equally as co-authors.
Tim N FordWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
Dongyao CuiWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
Robert W CarruthWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
Kanwarpal SinghWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
Linbo LiuWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
Susan E BirketDepartment of Medicine and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, MCLM 706, 1918 University Blvd, Birmingham, AL, 35294-0005, USA.
George M SolomonDepartment of Medicine and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, MCLM 706, 1918 University Blvd, Birmingham, AL, 35294-0005, USA.
Steven M RoweDepartment of Medicine and the Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, MCLM 706, 1918 University Blvd, Birmingham, AL, 35294-0005, USA; Contributed equally as senior authors.
Guillermo J TearneyWellman Center for Photomedicine, Department of Determatology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA; Contributed equally as senior authors.
Massachusetts General Hospital · USHarvard University · US

Funding

UAB CF Research and Translation Core CenterP30DK072482 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI David M. Bedwell · 2007 to 2026
$23.0M
Functional Anatomic Imaging of CF Patients with Early Lung Disease Using microOCTR01HL116213 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ROWE, STEVEN MARK, TEARNEY, GUILLERMO J · 2012 to 2015
$2.0M
NHLBI NIH HHS R01 HL116213NIDDK NIH HHS P30 DK072482
6 · The paper itself

Abstract

We have designed and fabricated a 4 mm diameter rigid endoscopic probe to obtain high resolution micro-optical coherence tomography (µOCT) images from the tracheal epithelium of living swine. Our common-path fiber-optic probe used gradient-index focusing optics, a selectively coated prism reflector to implement a circular-obscuration apodization for depth-of-focus enhancement, and a common-path reference arm and an ultra-broadbrand supercontinuum laser to achieve high axial resolution. Benchtop characterization demonstrated lateral and axial resolutions of 3.4 μm and 1.7 μm, respectively (in tissue). Mechanical standoff rails flanking the imaging window allowed the epithelial surface to be maintained in focus without disrupting mucus flow. During in vivo imaging, relative motion was mitigated by inflating an airway balloon to hold the standoff rails on the epithelium. Software implemented image stabilization was also implemented during post-processing. The resulting image sequences yielded co-registered quantitative outputs of airway surface liquid and periciliary liquid layer thicknesses, ciliary beat frequency, and mucociliary transport rate, metrics that directly indicate airway epithelial function that have dominated in vitro research in diseases such as cystic fibrosis, but have not been available in vivo.

Indexed as

(170.1610) Clinical applications(170.2150) Endoscopic imaging(170.4500) Optical coherence tomography(170.4580) Optical diagnostics for medicine

Identifiers

PMID27446685
PMCPMC4948609
OpenAlexW2419223796

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.