ArticleBiomedical optics express2016
Arduino Due based tool to facilitate in vivo two-photon excitation microscopy.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A modular and flexible open source cell incubator system for mobile and stationary use.HardwareX · 2024Article
- MicroFPGA: An affordable FPGA platform for microscope control.HardwareX · 2023Article
- Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter.Nature communications · 2020Article
- Reproducible Quantitative Stimulation Allows New Analysis of Crayfish Muscle Receptor Organ Responses.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2020Article
- Simultaneous two-photon imaging of intracellular chloride concentration and pH in mouse pyramidal neurons in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
5 authors.
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Abstract
Two-photon excitation spectroscopy is a powerful technique for the characterization of the optical properties of genetically encoded and synthetic fluorescent molecules. Excitation spectroscopy requires tuning the wavelength of the Ti:sapphire laser while carefully monitoring the delivered power. To assist laser tuning and the control of delivered power, we developed an Arduino Due based tool for the automatic acquisition of high quality spectra. This tool is portable, fast, affordable and precise. It allowed studying the impact of scattering and of blood absorption on two-photon excitation light. In this way, we determined the wavelength-dependent deformation of excitation spectra occurring in deep tissues in vivo.
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