Evidence map›Paper›PMID 27446675›Full record

ArticleBiomedical optics express2016

Measuring membrane association and protein diffusion within membranes with supercritical angle fluorescence microscopy.

Yuanqing Ma, Aleš Benda, Philip R Nicovich, Katharina Gaus

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 15 citations in OpenAlex.

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  6. Clustering of the ζ-Chain Can Initiate T Cell Receptor Signaling.International journal of molecular sciences · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Yuanqing MaEMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney 2052 Australia; ARC Center of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney 2052 Australia.
Aleš BendaBiomedical Imaging Facility, Lowy Cancer Research Center, University of New South Wales, High Street, Kensington, Sydney, NSW, 2052, Australia; IMCF at BIOCEV, Faculty of Science, Charles University in Prague, Czech Republic.
Philip R NicovichEMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney 2052 Australia; ARC Center of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney 2052 Australia.
Katharina GausEMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney 2052 Australia; ARC Center of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney 2052 Australia.
EMBL Australia · AUUNSW Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Supercritical angle fluorescence (SAF) detection combines the axial discrimination and exquisite signal-to-noise ratio of total internal reflection fluorescence (TIRF) with the lateral discrimination and convenience of confocal excitation. This combination makes SAF ideal for fluorescence correlation spectroscopy (FCS) on membranes and other structures in close proximity to the coverslip. Here we report a straightforward modification of a commercial microscope to implement SAF FCS and demonstrate in both model supported lipid bilayers and cellular systems that this approach shows an increase in signal from membrane-bound fluorophores relative to fluorophores in solution, benchmarked against line-scanning FCS. SAF FCS allowed us to demonstrate that activation of the T cell receptor resulted in the recruitment of the kinase Lck to the plasma membrane as well as a reduction in Lck mobility within the membrane.

Indexed as

(110.2945) Illumination design(110.2970) Image detection systems(300.6280) Spectroscopy, fluorescence and luminescence

Identifiers

PMID27446675
PMCPMC4929661
OpenAlexW2313531676

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.