ArticleTheranostics2016
Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum.
Article in Theranostics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 53 citations in OpenAlex.
- Harnessing Bioluminescent Bacteria to Develop an Enzymatic-free Enzyme-linked immunosorbent assay for the Detection of Clinically Relevant Biomarkers.ACS applied materials & interfaces · 2024Article
- SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury.RSC advances · 2022Article
- Recent advances on the development of plasmon-assisted biosensors for detection of C-reactive protein.Journal of molecular structure · 2021Article
- Reusable, Noninvasive, and Sensitive Fluorescence Enhanced ZnO-Nanorod-Based Microarrays for Quantitative Detection of AFP in Human Serum.BioMed research international · 2021Article
- Highly enhanced ELISA sensitivity using acetylated chitosan surfaces.BMC biotechnology · 2020Article
- A colorimetric method for determination of the prostate specific antigen based on enzyme-free cascaded signal amplification via peptide-copper(II) nanoparticles.Mikrochimica acta · 2020Article
- Recent Progress of Biomarker Detection Sensors.Research (Washington, D.C.) · 2020Review
- A quartz crystal microbalance modified with antibody-coated silver nanoparticles acting as mass signal amplifiers for real-time monitoring of three latent tuberculosis infection biomarkers.Mikrochimica acta · 2019Article
- Nanomaterials-Based Colorimetric Immunoassays.Nanomaterials (Basel, Switzerland) · 2019Review
- Comparative analysis for strength serum sodium and potassium in three different methods: Flame photometry, ion-selective electrode (ISE) and colorimetric enzymatic.Journal of clinical laboratory analysis · 2018Article
- An ultrasensitive flow cytometric immunoassay based on bead surface-initiated template-free DNA extension.Chemical science · 2018Article
- Enzyme-Free Immunoassay Using Silver Nanoparticles for Detection of Gliadin at Ultralow Concentrations.ACS omega · 2018Article
- ColorimetricTheranostics · 2018Article
- D-alanyl-D-alanine-Modified Gold Nanoparticles Form a Broad-Spectrum Sensor for Bacteria.Theranostics · 2018Article
- Development and Comparison of Two Immuno-disaggregation Based Bioassays for Cell Secretome Analysis.Theranostics · 2018Article
- Incorporating gold nanoclusters and target-directed liposomes as a synergistic amplified colorimetric sensor for HER2-positive breast cancer cell detection.Theranostics · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determination of disease biomarkers in clinical samples is of crucial significance for disease monitoring and public health. The dominating format is enzyme-linked immunosorbent assay (ELISA), which subtly exploits both the antigen-antibody reaction and biocatalytic property of enzymes. Although enzymes play an important role in this platform, they generally suffer from inferior stability and less tolerant of temperature, pH condition compared with general chemical product. Here, we demonstrate a metal-linked immunosorbent assay (MeLISA) based on a robust signal amplification mechanism that faithfully replaces the essential element of the enzyme. As an enzyme-free alternative to ELISA, this methodology works by the detection of α-fetoprotein (AFP), prostatic specific antigen (PSA) and C-reactive protein (CRP) at concentrations of 0.1 ng mL(-1), 0.1 ng mL(-1) and 1 ng mL(-1) respectively. It exhibits approximately two magnitudes higher sensitivity and is 4 times faster for chromogenic reaction than ELISA. The detection of AFP and PSA was further confirmed by over a hundred serum samples from hepatocellular carcinoma (HCC) and prostate cancer patients respectively.
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