ArticleFrontiers in plant science2016
Autofluorescence as a Signal to Sort Developing Glandular Trichomes by Flow Cytometry.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- The SlGRAS9-SlMYC1 regulatory module controls glandular trichome formation and modulates resilience to pest in tomato.The Plant journal : for cell and molecular biology · 2025Article
- Protocol to analyse the structural composition by fluorescence microscopy and different conventional and fluorescence staining methods.MethodsX · 2024Article
- Novel players in organogenesis and flavonoid biosynthesis in cucumber glandular trichomes.Plant physiology · 2023Article
- Glandular and Non-Glandular Trichomes fromPlants (Basel, Switzerland) · 2023Article
- Glandular trichomes: new focus on horticultural crops.Horticulture research · 2021Review
- Mediation of JA signalling in glandular trichomes by the woolly/SlMYC1 regulatory module improves pest resistance in tomato.Plant biotechnology journal · 2021Article
- Article
- Article
- Autofluorescence mediated red spherulocyte sorting provides insights into the source of spinochromes in sea urchins.Scientific reports · 2020Article
- Differences in the Structural Chemical Composition of the Primary Xylem of Cactaceae: A Topochemical Perspective.Frontiers in plant science · 2019Article
- Chemical fingerprinting of single glandular trichomes of Cannabis sativa by Coherent anti-Stokes Raman scattering (CARS) microscopy.BMC plant biology · 2018Article
- Article
- QTL Mapping of the Shape of Type VI Glandular Trichomes in Tomato.Frontiers in plant science · 2018Article
- Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest.Plant physiology · 2017Review
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The industrial relevance of a number of metabolites produced in plant glandular trichomes (GTs) has spurred research on these specialized organs for a number of years. Most of the research, however, has focused on the elucidation of secondary metabolite pathways and comparatively little has been undertaken on the development and differentiation of GTs. One way to gain insight into these developmental processes is to generate stage-specific transcriptome and metabolome data. The difficulty for this resides in the isolation of early stages of development of the GTs. Here we describe a method for the separation and isolation of intact young and mature type VI trichomes from the wild tomato species Solanum habrochaites. The final and key step of the method uses cell sorting based on distinct autofluorescence signals of the young and mature trichomes. We demonstrate that sorting by flow cytometry allows recovering pure fractions of young and mature trichomes. Furthermore, we show that the sorted trichomes can be used for transcript and metabolite analyses. Because many plant tissues or cells have distinct autofluorescence components, the principles of this method can be generally applicable for the isolation of specific cell types without prior labeling.
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