ArticleFrontiers in plant science2016
De novo Transcriptome Sequencing to Dissect Candidate Genes Associated with Pearl Millet-Downy Mildew (Sclerospora graminicola Sacc.) Interaction.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Modern genomic and omics-based technologies for millet breeding and genetic improvement.Frontiers in plant science · 2026Review
- Genome-wide identification and expression analysis ofPlant signaling & behavior · 2025Article
- When genes turn traitor:Frontiers in plant science · 2025Article
- Pearl millet a promising fodder crop for changing climate: a review.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- QTL mapping of downy mildew resistance in foxtail millet by SLAF‑seq and BSR-seq analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- LMTFrontiers in plant science · 2023Article
- Comparative transcriptome profiling of resistant and susceptible foxtail millet responses to Sclerospora graminicola infection.BMC plant biology · 2022Article
- Identification of genes controlling compatible and incompatible reactions of pearl millet (Frontiers in plant science · 2022Article
- Integration of early disease-resistance phenotyping, histological characterization, and transcriptome sequencing reveals insights into downy mildew resistance in impatiens.Horticulture research · 2021Article
- QTL-seq analysis of powdery mildew resistance in a Korean cucumber inbred line.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Pearl Millet: A Climate-Resilient Nutricereal for Mitigating Hidden Hunger and Provide Nutritional Security.Frontiers in plant science · 2021Review
- Transcriptome analysis for molecular landscaping of genes controlling diterpene andrographolide biosynthesis in3 Biotech · 2020Article
- Transcriptome analysis of heat stress and drought stress in pearl millet based on Pacbio full-length transcriptome sequencing.BMC plant biology · 2020Article
- Identification and Validation of Candidate Genes Conferring Resistance to Downy Mildew in Maize (Genes · 2020Article
- Article
- PK-profiling method for identifying the expression of resistance-associated genes in partially resistant oats to crown rust.BMC plant biology · 2018Article
- Transcriptomic profiling of developing fiber in levant cotton (Gossypium herbaceum L.).Functional & integrative genomics · 2018Article
- Genome analysis of the foxtail millet pathogen Sclerospora graminicola reveals the complex effector repertoire of graminicolous downy mildews.BMC genomics · 2017Article
- RNA-seq of Rice Yellow Stem BorerG3 (Bethesda, Md.) · 2017Article
- Exploration of Genetic and Genomic Resources for Abiotic and Biotic Stress Tolerance in Pearl Millet.Frontiers in plant science · 2016Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the plant-pathogen interactions is of utmost importance to design strategies for minimizing the economic deficits caused by pathogens in crops. With an aim to identify genes underlying resistance to downy mildew, a major disease responsible for productivity loss in pearl millet, transcriptome analysis was performed in downy mildew resistant and susceptible genotypes upon infection and control on 454 Roche NGS platform. A total of ~685 Mb data was obtained with 1 575 290 raw reads. The raw reads were pre-processed into high-quality (HQ) reads making to ~82% with an average of 427 bases. The assembly was optimized using four assemblers viz. Newbler, MIRA, CLC and Trinity, out of which MIRA with a total of 14.10 Mb and 90118 transcripts proved to be the best for assembling reads. Differential expression analysis depicted 1396 and 936 and 1000 and 1591 transcripts up and down regulated in resistant inoculated/resistant control and susceptible inoculated/susceptible control respectively with a common of 3644 transcripts. The pathways for secondary metabolism, specifically the phenylpropanoid pathway was up-regulated in resistant genotype. Transcripts up-regulated as a part of defense response included classes of R genes, PR proteins, HR induced proteins and plant hormonal signaling transduction proteins. The transcripts for skp1 protein, purothionin, V type proton ATPase were found to have the highest expression in resistant genotype. Ten transcripts, selected on the basis of their involvement in defense mechanism were validated with qRT-PCR and showed positive co-relation with transcriptome data. Transcriptome analysis evoked potentials of hypersensitive response and systemic acquired resistance as possible mechanism operating in defense mechanism in pearl millet against downy mildew infection.
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