ArticleFrontiers in microbiology2016
Insights into Abundant Rumen Ureolytic Bacterial Community Using Rumen Simulation System.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 85 citations in OpenAlex.
- Animal gut microbes and microbiomes in the 21st century and beyond.Science China. Life sciences · 2026Review
- Diversity, Composition, and Co-occurrence Network of Rhizosphere and Endophytic Bacterial Communities in Two Growth Stages of Leonurus japonicus.Current microbiology · 2025Article
- Effects of Soybean Meal Replacement on Growth Performance, Rumen Fermentation, Rumen Microorganisms, and Metabolites in Dumont Lambs.Animals : an open access journal from MDPI · 2025Article
- Cellulose-functionalized magnetic nanoparticle-mediated isolation of novel cellulolytic bacteria.Applied and environmental microbiology · 2025Article
- The role of milk urea nitrogen in nutritional assessment and its relationship with phenotype of dairy cows: A review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Review
- Exploring the Linkage Between Ruminal Microbial Communities on Postweaning and Finishing Diets and Their Relation to Residual Feed Intake in Beef Cattle.Microorganisms · 2024Article
- Hydrogen-rich water 400ppb as a potential strategy for improving ruminant nutrition and mitigating methane emissions.BMC microbiology · 2024Article
- Effects of biochanin A on lactational performance, nitrogen metabolism, and blood metabolites in dairy cows.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Assessing the impact of three feeding stages on rumen bacterial community and physiological characteristics of Japanese Black cattle.Scientific reports · 2024Article
- Core microbiota for nutrient digestion remained and ammonia utilization increased after continuous batch culture of rumen microbiotaFrontiers in microbiology · 2024Article
- Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration usingFrontiers in veterinary science · 2024Article
- Multi-omics revealed the long-term effect of ruminal keystone bacteria and the microbial metabolome on lactation performance in adult dairy goats.Microbiome · 2023Article
- Functional gene-guided enrichment plus in situ microsphere cultivation enables isolation of new crucial ureolytic bacteria from the rumen of cattle.Microbiome · 2023Article
- Potential roles of the rectum keystone microbiota in modulating the microbial community and growth performance in goat model.Journal of animal science and biotechnology · 2023Article
- Microbial Populations in Ruminal Liquid Samples from Young Beefmaster Bulls at Both Extremes of RFI Values.Microorganisms · 2023Article
- Isolation and pan-genome analysis ofFrontiers in microbiology · 2023Article
- Gut symbiotic bacteria are involved in nitrogen recycling in the tephritid fruit fly Bactrocera dorsalis.BMC biology · 2022Article
- Understanding the role of rumen epithelial host-microbe interactions in cattle feed efficiency.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Review
- Activity- and Enrichment-Based Metaproteomics Insights into Active Urease from the Rumen Microbiota of Cattle.International journal of molecular sciences · 2022Article
- Heat Stress: Effects on Rumen Microbes and Host Physiology, and Strategies to Alleviate the Negative Impacts on Lactating Dairy Cows.Frontiers in microbiology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However, there is limited knowledge with regard to the ureolytic bacteria community in the rumen. To explore the ruminal ureolytic bacterial community, urea, or acetohydroxamic acid (AHA, an inhibitor of urea hydrolysis) were supplemented into the rumen simulation systems. The bacterial 16S rRNA genes were sequenced by Miseq high-throughput sequencing and used to reveal the ureoltyic bacteria by comparing different treatments. The results revealed that urea supplementation significantly increased the ammonia concentration, and AHA addition inhibited urea hydrolysis. Urea supplementation significantly increased the richness of bacterial community and the proportion of ureC genes. The composition of bacterial community following urea or AHA supplementation showed no significant difference compared to the groups without supplementation. The abundance of Bacillus and unclassified Succinivibrionaceae increased significantly following urea supplementation. Pseudomonas, Haemophilus, Neisseria, Streptococcus, and Actinomyces exhibited a positive response to urea supplementation and a negative response to AHA addition. Results retrieved from the NCBI protein database and publications confirmed that the representative bacteria in these genera mentioned above had urease genes or urease activities. Therefore, the rumen ureolytic bacteria were abundant in the genera of Pseudomonas, Haemophilus, Neisseria, Streptococcus, Actinomyces, Bacillus, and unclassified Succinivibrionaceae. Insights into abundant rumen ureolytic bacteria provide the regulation targets to mitigate urea hydrolysis and increase efficiency of urea nitrogen utilization in ruminants.
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