ArticleFrontiers in microbiology2016
Identification of the agr Peptide of Listeria monocytogenes.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- EngineeredBiochemistry · 2026Article
- Adaptive Tolerance of Listeria monocytogenes to Chemical Oxidants: Comparative Analysis of Transcriptomic Studies.Comprehensive reviews in food science and food safety · 2025Review
- A short-lived peptide signal regulates cell-to-cell communication in Listeria monocytogenes.Communications biology · 2024Article
- Cross-Phosphorylation between AgrC Histidine Kinase and the Noncognate Response Regulator Lmo1172 inMicroorganisms · 2024Article
- Characterization of an Autoinducing Peptide Signal Reveals Highly Efficacious Synthetic Inhibitors and Activators of Quorum Sensing and Biofilm Formation inBiochemistry · 2023Article
- Cinnamaldehyde Targets the LytTR DNA-Binding Domain of the Response Regulator AgrA to Attenuate Biofilm Formation of Listeria monocytogenes.Microbiology spectrum · 2023Article
- Antibiofilm, AntiAdhesive and Anti-Invasive Activities of Bacterial Lysates Extracted fromFoods (Basel, Switzerland) · 2022Article
- Article
- Andrographolide Inhibits Biofilm and Virulence inMolecules (Basel, Switzerland) · 2022Article
- Review
- Enzyme-Primed Native Chemical Ligation Produces Autoinducing Cyclopeptides in Clostridia.Angewandte Chemie (International ed. in English) · 2021Article
- Quorum quenching enzymes and their effects on virulence, biofilm, and microbiomes: a review of recent advances.Expert review of anti-infective therapy · 2020Review
- Development and utilization of peptide-based quorum sensing modulators in Gram-positive bacteria.Organic & biomolecular chemistry · 2020Review
- Alternative approaches to treat bacterial infections: targeting quorum-sensing.Expert review of anti-infective therapy · 2020Review
- Links between S-adenosylmethionine and Agr-based quorum sensing for biofilm development in Listeria monocytogenes EGD-e.MicrobiologyOpen · 2020Article
- The manifold roles of microbial ribosomal peptide-based natural products in physiology and ecology.The Journal of biological chemistry · 2020Review
- Inhibition of Biofilm Formation and Related Gene Expression ofFrontiers in microbiology · 2020Article
- Efficacy of Synthetic Furanones onFoods (Basel, Switzerland) · 2019Article
- Characterization of the biofilm phenotype of a Listeria monocytogenes mutant deficient in agr peptide sensing.MicrobiologyOpen · 2019Article
- Regulation ofMicrobiology spectrum · 2019Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes (Lm) is an important food-borne human pathogen that is able to strive under a wide range of environmental conditions. Its accessory gene regulator (agr) system was shown to impact on biofilm formation and virulence and has been proposed as one of the regulatory mechanisms involved in adaptation to these changing environments. The Lm agr operon is homologous to the Staphylococcus aureus system, which includes an agrD-encoded autoinducing peptide that stimulates expression of the agr genes via the AgrCA two-component system and is required for regulation of target genes. The aim of the present study was to identify the native autoinducing peptide (AIP) of Lm using a luciferase reporter system in wildtype and agrD deficient strains, rational design of synthetic peptides and mass spectrometry. Upon deletion of agrD, luciferase reporter activity driven by the PII promoter of the agr operon was completely abolished and this defect was restored by co-cultivation of the agrD-negative reporter strain with a producer strain. Based on the sequence and structures of known AIPs of other organisms, a set of potential Lm AIPs was designed and tested for PII-activation. This led to the identification of a cyclic pentapeptide that was able to induce PII-driven luciferase reporter activity and restore defective invasion of the agrD deletion mutant into Caco-2 cells. Analysis of supernatants of a recombinant Escherichia coli strain expressing AgrBD identified a peptide identical in mass and charge to the cyclic pentapeptide. The Lm agr system is specific for this pentapeptide since the AIP of Lactobacillus plantarum, which also is a pentapeptide yet with different amino acid sequence, did not induce PII activity. In summary, the presented results provide further evidence for the hypothesis that the agrD gene of Lm encodes a secreted AIP responsible for autoregulation of the agr system of Lm. Additionally, the structure of the native Lm AIP was identified.
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