ArticleArteriosclerosis, thrombosis, and vascular biology2016
MYOSLID Is a Novel Serum Response Factor-Dependent Long Noncoding RNA That Amplifies the Vascular Smooth Muscle Differentiation Program.
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Article in Arteriosclerosis, thrombosis, and vascular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
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75citing papers in PubMed, 1 pooled it
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75 citing papers in PubMed, 1 synthesis or guideline pooled it, 108 citations in OpenAlex.
- Targeting epigenetics and non-coding RNAs in atherosclerosis: from mechanisms to therapeutics.Pharmacology & therapeutics · 2019Pooled it
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- MAPK14 converges on key transcriptional machinery to promote vascular smooth muscle cell degeneration in abdominal aortic aneurysm.Signal transduction and targeted therapy · 2026Article
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15 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 3 institutions in 1 country.
Jinjing ZhaoFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Wei ZhangFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Mingyan LinFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Wen WuFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Pengtao JiangFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Emiley TouFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Min XueFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Angelene RichardsFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
David Jourd'heuilFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Arif AsifFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Deyou ZhengFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Harold A SingerFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Joseph M MianoFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.).
Xiaochun LongFrom the Department of Molecular and Cellular Physiology (J.Z., W.Z., W.W., E.T., M.X., A.R., D.J., H.A.S., X.L.), Albany Medical College, NY; Department of Medicine, Jersey Shore University Medical Center, Neptune, NJ (A.A.); Departments of Genetics (M.L., D.Z.) and Neurology and Neuroscience (D.Z.), Albert Einstein College of Medicine, Bronx, NY; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (P.J., J.M.M.); and National Aquafeed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (M.X.). longx@mail.amc.edu.
Albert Einstein College of Medicine · USUniversity of Rochester · USJersey Shore University Medical Center · US
Funding
CALCIUM/CALMODULIN ACTIVATED KINIASES IN SMOOTH MUSCLER01HL049426 · NHLBI · WEIS CENTER FOR RESEARCH-GEISINGER CLINC · PI SINGER, HAROLD A · 1994 to 2019
$6.2MNovel Role of MAPK14 in Regulation of VSMC Contractile PhenotypeR01HL122686 · NHLBI · AUGUSTA UNIVERSITY · PI LONG, XIAOCHUN · 2014 to 2022
$4.0MRegulation and Function of Myocardin in Vascular PathobiologyR01HL117907 · NHLBI · UNIVERSITY OF ROCHESTER · PI MIANO, JOSEPH M · 2013 to 2016
$1.5MNHLBI NIH HHS R01 HL049426NHLBI NIH HHS R01 HL117907NHLBI NIH HHS R01 HL122686
Abstract
objectiveLong noncoding RNAs (lncRNA) represent a growing class of noncoding genes with diverse cellular functions. We previously reported on SENCR, an lncRNA that seems to support the vascular smooth muscle cell (VSMC) contractile phenotype. However, information about the VSMC-specific lncRNAs regulated by myocardin (MYOCD)/serum response factor, the master switch for VSMC differentiation, is unknown. APPROACH AND
resultsTo define novel lncRNAs with functions related to VSMC differentiation, we performed RNA sequencing in human coronary artery SMCs that overexpress MYOCD. Several novel lncRNAs showed altered expression with MYOCD overexpression and one, named MYOcardin-induced Smooth muscle LncRNA, Inducer of Differentiation (MYOSLID), was activated by MYOCD and selectively expressed in VSMCs. MYOSLID was a direct transcriptional target of both MYOCD/serum response factor and transforming growth factor-β/SMAD pathways. Functional studies revealed that MYOSLID promotes VSMC differentiation and inhibits VSMC proliferation. MYOSLID showed reduced expression in failed human arteriovenous fistula samples compared with healthy veins. Although MYOSLID did not affect gene expression of transcription factors, such as serum response factor and MYOCD, its depletion in VSMCs disrupted actin stress fiber formation and blocked nuclear translocation of MYOCD-related transcription factor A (MKL1). Finally, loss of MYOSLID abrogated transforming growth factor-β1-induced SMAD2 phosphorylation.
conclusionsWe have demonstrated that MYOSLID, the first human VSMC-selective and serum response factor/CArG-dependent lncRNA, is a novel modulator in amplifying the VSMC differentiation program, likely through feed-forward actions of both MKL1 and transforming growth factor-β/SMAD pathways.
Indexed as
Cell DifferentiationMuscle DevelopmentActive Transport, Cell NucleusArteriovenous Shunt, SurgicalCell ProliferationCells, CulturedCoronary VesselsGene Expression RegulationHumansHuman Umbilical Vein Endothelial CellsMuscle, Smooth, VascularMyocardinMyocytes, Smooth MuscleNuclear ProteinsPhenotypePhosphorylationlong non-coding RNA MYOSLID, humanMRTFA protein, humanMyocardinNuclear ProteinsRNA, Long NoncodingSerum Response FactorSmad2 ProteinSMAD2 protein, humanTGFB1 protein, humanTrans-ActivatorsTransforming Growth Factor beta1long noncoding RNAmyocardinphenotypestress fibervascular smooth muscle
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