ArticleThe Journal of infectious diseases2016
Dynamics of Dengue Virus (DENV)-Specific B Cells in the Response to DENV Serotype 1 Infections, Using Flow Cytometry With Labeled Virions.
Article in The Journal of infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Virus-like Particles and Spectral Flow Cytometry for Identification of Dengue Virus-Specific B Cells in Mice and Humans.Viruses · 2025Article
- The global landscape of approved antibody therapies.Antibody therapeutics · 2022Review
- Dengue Infection - Recent Advances in Disease Pathogenesis in the Era of COVID-19.Frontiers in immunology · 2022Review
- Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.Cell reports. Medicine · 2021Article
- Adaptive Immunity to Dengue Virus: Slippery Slope or Solid Ground for Rational Vaccine Design?Pathogens (Basel, Switzerland) · 2020Review
- Development of therapeutic antibodies for the treatment of diseases.Journal of biomedical science · 2020Review
- Approaches to Interrogating the Human Memory B-Cell and Memory-Derived Antibody Repertoire Following Dengue Virus Infection.Frontiers in immunology · 2019Review
- Multiplexed FluoroSpot for the Analysis of Dengue Virus- and Zika Virus-Specific and Cross-Reactive Memory B Cells.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Protective versus pathologic pre-exposure cytokine profiles in dengue virus infection.PLoS neglected tropical diseases · 2018Article
- Activation of Peripheral T Follicular Helper Cells During Acute Dengue Virus Infection.The Journal of infectious diseases · 2018Article
- Isolation and Characterization of Antigen-Specific Plasmablasts Using a Novel Flow Cytometry-Based Ig Capture Assay.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Detection, phenotyping and quantification of dengue virus-specific B cells using fluorescent probes.Human vaccines & immunotherapeutics · 2017Article
- Humanized mouse models to study human cell-mediated and humoral responses to dengue virus.Current opinion in virology · 2017Review
- Immune-mediated cytokine storm and its role in severe dengue.Seminars in immunopathology · 2017Review
- Dengue virus antibody database: Systematically linking serotype-specificity with epitope mapping in dengue virus.PLoS neglected tropical diseases · 2017Article
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9 authors.
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Abstract
backgroundThe development of reagents to identify and characterize antigen-specific B cells has been challenging.
methodsWe recently developed Alexa Fluor-labeled dengue viruses (AF DENVs) to characterize antigen-specific B cells in the peripheral blood of DENV-immune individuals.
resultsIn this study, we used AF DENV serotype 1 (AF DENV-1) together with AF DENV-2 on peripheral blood mononuclear cells (PBMCs) from children in Thailand with acute primary or secondary DENV-1 infections to analyze the phenotypes of antigen-specific B cells that reflected their exposure or clinical diagnosis. DENV serotype-specific and cross-reactive B cells were identified in PBMCs from all subjects. Frequencies of AF DENV(+) class-switched memory B cells (IgD(-)CD27(+) CD19(+) cells) reached up to 8% during acute infection and early convalescence. AF DENV-labeled B cells expressed high levels of CD27 and CD38 during acute infection, characteristic of plasmablasts, and transitioned into memory B cells (CD38(-)CD27(+)) at the early convalescent time point. There was higher activation of memory B cells early during acute secondary infection, suggesting reactivation from a previous DENV infection.
conclusionsAF DENVs reveal changes in the phenotype of DENV serotype-specific and cross-reactive B cells during and after natural DENV infection and could be useful in analysis of the response to DENV vaccination.
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