ArticlePlant physiology2016
Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Compensatory respiration supports survival during extended heat stress inScience advances · 2026Article
- A PSII photosynthetic control is activated in anoxic cultures of green algae following illumination.Communications biology · 2023Article
- The relationship between photosystem II regulation and light-dependent hydrogen production by microalgae.Biophysical reviews · 2022Review
- Synthetic biology for improved hydrogen production in Chlamydomonas reinhardtii.Microbial biotechnology · 2022Review
- Photosystem I light-harvesting proteins regulate photosynthetic electron transfer and hydrogen production.Plant physiology · 2022Article
- Article
- Phylloquinone is the principal Mehler reaction site within photosystem I in high light.Plant physiology · 2021Article
- Water oxidation by photosystem II is the primary source of electrons for sustained HProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Overproduction of the Flv3B flavodiiron, enhances the photobiological hydrogen production by the nitrogen-fixing cyanobacterium Nostoc PCC 7120.Microbial cell factories · 2020Article
- Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research.Frontiers in plant science · 2020Article
- Mechanistic insights into pH-dependent HBiotechnology for biofuels · 2020Article
- Elimination of the flavodiiron electron sink facilitates long-term HBiotechnology for biofuels · 2019Article
- Flavodiiron-Mediated OPlant physiology · 2018Article
- Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen.Photosynthesis research · 2018Article
- Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place.Plant physiology · 2018Article
- Water-splitting-based, sustainable and efficient HBiotechnology for biofuels · 2018Article
- Genetically modified algae produce hydrogen biofuel at five times their normal yield.Science progress · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photosynthetic hydrogen production in the microalga Chlamydomonas reinhardtii is catalyzed by two [FeFe]-hydrogenase isoforms, HydA1 and HydA2, both irreversibly inactivated upon a few seconds exposure to atmospheric oxygen. Until recently, it was thought that hydrogenase is not active in air-grown microalgal cells. In contrast, we show that the entire pool of cellular [FeFe]-hydrogenase remains active in air-grown cells due to efficient scavenging of oxygen. Using membrane inlet mass spectrometry, (18)O2 isotope, and various inhibitors, we were able to dissect the various oxygen uptake mechanisms. We found that both chlororespiration, catalyzed by plastid terminal oxidase, and Mehler reactions, catalyzed by photosystem I and Flavodiiron proteins, significantly contribute to oxygen uptake rate. This rate is considerably enhanced with increasing light, thus forming local anaerobic niches at the proximity of the stromal face of the thylakoid membrane. Furthermore, we found that in transition to high light, the hydrogen production rate is significantly enhanced for a short duration (100 s), thus indicating that [FeFe]-hydrogenase functions as an immediate sink for surplus electrons in aerobic as well as in anaerobic environments. In summary, we show that an anaerobic locality in the chloroplast preserves [FeFe]-hydrogenase activity and supports continuous hydrogen production in air-grown microalgal cells.
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