ArticleDiabetologia2016
Sequential intravital imaging reveals in vivo dynamics of pancreatic tissue transplanted under the kidney capsule in mice.
Article in Diabetologia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Comparative analysis of dose-response variability and severity in STZ-induced diabetes: female vs. male NSG mice.Scientific reports · 2026Article
- Stable intracranial imaging of dura mater-engrafted pancreatic islet cells in awake mice.Nature communications · 2025Article
- Longitudinal intravital microscopy of the mouse kidney: inflammatory responses to abdominal imaging windows.American journal of physiology. Renal physiology · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Mouse models and human islet transplantation sites for intravital imaging.Frontiers in endocrinology · 2022Review
- Optoacoustic Imaging of Glucagon-like Peptide-1 Receptor with a Near-Infrared Exendin-4 Analog.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2021Article
- State-of-the-art microscopy to understand islets of Langerhans: what to expect next?Immunology and cell biology · 2021Review
- Functional analysis of islet cells in vitro, in situ, and in vivo.Seminars in cell & developmental biology · 2020Review
- Article
- A Novel Pancreatic Imaging Window for Stabilized LongitudinalDiabetes & metabolism journal · 2020Article
- Noninvasive intravital high-resolution imaging of pancreatic neuroendocrine tumours.Scientific reports · 2019Article
- A Versatile, Portable Intravital Microscopy Platform for Studying Beta-cell Biology In Vivo.Scientific reports · 2019Article
- A Bright Future for Fluorescence Imaging of Fungi in Living Hosts.Journal of fungi (Basel, Switzerland) · 2019Review
- Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo.Stem cell reports · 2018Article
- The allosteric AKT inhibitor, MK2206, decreases tumor growth and invasion in patient derived xenografts of endometrial cancer.Cancer biology & therapy · 2017Article
- Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs.Methods (San Diego, Calif.) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisDynamic processes in pancreatic tissue are difficult to study. We aimed to develop an intravital imaging method to longitudinally examine engraftment, vascularisation, expansion and differentiation in mature islets or embryonic pancreases transplanted under the kidney capsule.
methodsIsolated pancreatic islets from adult mice and murine embryonic day (E)12.5 pancreases containing fluorescent biomarkers were transplanted under the kidney capsule of immunodeficient recipient mice. Human islet cells were dispersed, transduced with a lentivirus expressing a fluorescent label and reaggregated before transplantation. Graft-containing kidneys were positioned subcutaneously and an imaging window was fitted into the skin on top of the kidney. Intravital imaging using multiphoton microscopy was performed for up to 2 weeks. Volumes of fluorescently labelled cells were determined as a measure of development and survival.
resultsTransplanted islets and embryonic pancreases showed good engraftment and remained viable. Engraftment and vascularisation could be longitudinally examined in murine and human islet cells. Murine islet beta cell volume was unchanged over time. Transplanted embryonic pancreases increased to up to 6.1 times of their original volume and beta cell volume increased 90 times during 2 weeks. CONCLUSIONS/
interpretationThis method allows for repeated intravital imaging of grafts containing various sources of pancreatic tissue transplanted under the kidney capsule. Using fluorescent markers, dynamic information concerning engraftment or differentiation can be visualised and measured.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.