ArticleJournal of experimental botany2016
Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- Structural and functional comparison of hemoglobin Glb2-1 of Lotus japonicus with Glb1-1 and leghemoglobins.Journal of experimental botany · 2026Article
- CPOP1 is a key enzyme required for nodule microenvironment control and successful symbiotic nitrogen fixation in Lotus japonicus.Journal of integrative plant biology · 2025Article
- Dynamics of hemoglobins during nodule development, nitrate response, and dark stress in Lotus japonicus.Journal of experimental botany · 2024Article
- Nitric Oxide and Globin Glb1 RegulateAntioxidants (Basel, Switzerland) · 2023Article
- Control of the rhizobium-legume symbiosis by the plant nitrogen demand is tightly integrated at the whole plant level and requires inter-organ systemic signaling.Frontiers in plant science · 2023Review
- Signaling by reactive molecules and antioxidants in legume nodules.The New phytologist · 2022Review
- Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1.Journal of experimental botany · 2021Article
- Nitric oxide, crosstalk with stress regulators and plant abiotic stress tolerance.Plant cell reports · 2021Review
- Characteristics and Research Progress of Legume Nodule Senescence.Plants (Basel, Switzerland) · 2021Review
- Expression of soybean plant hemoglobin gene family under abiotic stresses.Plant biotechnology (Tokyo, Japan) · 2021Article
- Elevated Nitrogen Priming Induced Oxinitro-Responses and Water Deficit Tolerance in Rice.Plants (Basel, Switzerland) · 2021Article
- Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus.Microbes and environments · 2021Article
- Medicago truncatula Phytoglobin 1.1 controls symbiotic nodulation and nitrogen fixation via the regulation of nitric oxide concentration.The New phytologist · 2020Article
- Reactive Sulfur Species Interact with Other Signal Molecules in Root Nodule Symbiosis inAntioxidants (Basel, Switzerland) · 2020Article
- The Role of Nitric Oxide in Nitrogen Fixation by Legumes.Frontiers in plant science · 2020Review
- Plant Nitrate Reductases Regulate Nitric Oxide Production and Nitrogen-Fixing Metabolism During theFrontiers in plant science · 2020Article
- Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules ofAntioxidants (Basel, Switzerland) · 2019Article
- Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis.Plant, cell & environment · 2019Article
- Redefining nitric oxide production in legume nodules through complementary insights from electron paramagnetic resonance spectroscopy and specific fluorescent probes.Journal of experimental botany · 2018Article
- Potential of Rice Stubble as a Reservoir of Bradyrhizobial Inoculum in Rice-Legume Crop Rotation.Applied and environmental microbiology · 2017Article
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12 authors at 3 institutions in 3 countries.
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Abstract
Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia-legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5'-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels.
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