ArticlePlant biotechnology journal2017
ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 347 papers, 1 of them a synthesis that pooled it.
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Who cites it
347 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Chickpea Biofortification for Cytokinin DehydrogenaseFrontiers in genetics · 2022Pooled it
- Biochemical Adaptations ofPlant-environment interactions (Hoboken, N.J.) · 2026Review
- Abscisic Acid-Mediated Drought Tolerance inCurrent issues in molecular biology · 2026Review
- Bridging the bench-to-field gap: a quantitative multi-omics and CRISPR/Cas9 framework for climate-resilient agriculture.Transgenic research · 2026Review
- Review
- A review of ARGOS-mediated regulation of plant organ size: molecular mechanisms, environmental interactions, and agricultural implications.Functional & integrative genomics · 2026Review
- Article
- Nanotechnology-Enabled CRISPR Delivery: Emerging Opportunities in Agriculture and Forest Biotechnology.Plants (Basel, Switzerland) · 2026Review
- Water Stress and Shade Elicit Similar Convergent Responses in Maize Kernel Development and Gene Expression During Early Post-Pollination.Plants (Basel, Switzerland) · 2026Article
- Molecular Crosstalk Between Flowering Time and Drought Adaptation in Cereal Crops.Plants (Basel, Switzerland) · 2026Review
- Effects of CRISPR technology on agricultural sustainability: global applications and turkish perspective.Transgenic research · 2026Review
- Crops exposed to extreme conditions: perspectives of gene editing to improve stress tolerance.Plant cell reports · 2026Review
- Intermolecular-Interaction-Driven Adaptive Remodeling: A Network Perspective on Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2026Review
- Compact Cas12 Systems for Multiplex Engineering of Plant Abiotic-Stress Networks.Advanced genetics (Hoboken, N.J.) · 2026Article
- Beyond CRISPR/Cas9: emerging genome editing technologies for next-generation crop improvement.Molecular biology reports · 2026Review
- Breeding of a Multifoliolate Alfalfa Population Using CRISPR/Cas9-Generated Mutants and Evaluation of Agronomic Traits and Nutritive Value.Plants (Basel, Switzerland) · 2026Article
- Harnessing Antioxidants for Abiotic Stress Management: Mechanistic Insights and Prospects for Sustainable Agriculture.Antioxidants (Basel, Switzerland) · 2026Review
- Advances in CRISPR/Cas systems for engineering abiotic stress tolerance in plants: mechanisms and future prospects.Planta · 2026Review
- Transgenic Tobacco Plants Expressing Synthetic Peptides: A Functional and Structural Analysis for Pathogen Resistance.Plant biotechnology journal · 2026Article
- Genome-Edited Maize Expressing Two Native Genes Confers Broad-Spectrum Resistance to Northern Corn Leaf Blight.Molecular plant pathology · 2026Article
287 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize ARGOS8 is a negative regulator of ethylene responses. A previous study has shown that transgenic plants constitutively overexpressing ARGOS8 have reduced ethylene sensitivity and improved grain yield under drought stress conditions. To explore the targeted use of ARGOS8 native expression variation in drought-tolerant breeding, a diverse set of over 400 maize inbreds was examined for ARGOS8 mRNA expression, but the expression levels in all lines were less than that created in the original ARGOS8 transgenic events. We then employed a CRISPR-Cas-enabled advanced breeding technology to generate novel variants of ARGOS8. The native maize GOS2 promoter, which confers a moderate level of constitutive expression, was inserted into the 5'-untranslated region of the native ARGOS8 gene or was used to replace the native promoter of ARGOS8. Precise genomic DNA modification at the ARGOS8 locus was verified by PCR and sequencing. The ARGOS8 variants had elevated levels of ARGOS8 transcripts relative to the native allele and these transcripts were detectable in all the tissues tested, which was the expected results using the GOS2 promoter. A field study showed that compared to the WT, the ARGOS8 variants increased grain yield by five bushels per acre under flowering stress conditions and had no yield loss under well-watered conditions. These results demonstrate the utility of the CRISPR-Cas9 system in generating novel allelic variation for breeding drought-tolerant crops.
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