ArticlePloS one2016
Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Can Precisely Discriminate the Lineages of Listeria monocytogenes and Species of Listeria.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Identification and Differentiation of Non-HemolyticMolecules (Basel, Switzerland) · 2025Article
- Proteotyping ofMicroorganisms · 2023Article
- Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains ofMolecules (Basel, Switzerland) · 2022Article
- Review
- Isolation ofBiology · 2022Article
- Differentiation ofMicroorganisms · 2022Article
- Proteomic Characterization of Antibiotic Resistance inInternational journal of molecular sciences · 2021Article
- Protein Biomarker Identification for the Discrimination ofFrontiers in microbiology · 2021Article
- Differentiation ofEuropean journal of microbiology & immunology · 2019Article
- Proteotyping as alternate typing method to differentiate Campylobacter coli clades.Scientific reports · 2019Article
- Ribosomal background of the Bacillus cereus group thermotypes.Scientific reports · 2017Article
- Article
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4 authors.
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Abstract
The genetic lineages of Listeria monocytogenes and other species of the genus Listeria are correlated with pathogenesis in humans. Although matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has become a prevailing tool for rapid and reliable microbial identification, the precise discrimination of Listeria species and lineages remains a crucial issue in clinical settings and for food safety. In this study, we constructed an accurate and reliable MS database to discriminate the lineages of L. monocytogenes and the species of Listeria (L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii, L. grayi, and L. rocourtiae) based on the S10-spc-alpha operon gene encoded ribosomal protein mass spectrum (S10-GERMS) proteotyping method, which relies on both genetic information (genomics) and observed MS peaks in MALDI-TOF MS (proteomics). The specific set of eight biomarkers (ribosomal proteins L24, L6, L18, L15, S11, S9, L31 type B, and S16) yielded characteristic MS patterns for the lineages of L. monocytogenes and the different species of Listeria, and led to the construction of a MS database that was successful in discriminating between these organisms in MALDI-TOF MS fingerprinting analysis followed by advanced proteotyping software Strain Solution analysis. We also confirmed the constructed database on the proteotyping software Strain Solution by using 23 Listeria strains collected from natural sources.
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