ArticlePloS one2016
Probing the Rare Biosphere of the North-West Mediterranean Sea: An Experiment with High Sequencing Effort.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Extensive data mining uncovers novel diversity among members of the rare biosphere within the Thermoplasmatota.Microbiome · 2025Article
- Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability.Microbiology spectrum · 2023Article
- Top abundant deep ocean heterotrophic bacteria can be retrieved by cultivation.ISME communications · 2023Article
- Seasonal microbial dynamics in the ocean inferred from assembled and unassembled data: a view on the unknown biosphere.ISME communications · 2022Article
- Culturable Bacterial Diversity from the Basaltic Subsurface of the Young Volcanic Island of Surtsey, Iceland.Microorganisms · 2022Article
- Insights Into the Cultivable Bacterial Fraction of Sediments From the Red Sea Mangroves and Physiological, Chemotaxonomic, and Genomic Characterization ofFrontiers in microbiology · 2022Article
- Microbial diversity in tropical marine sediments assessed using culture-dependent and culture-independent techniques.Environmental microbiology · 2021Article
- Dynamic prokaryotic communities in the dark western Mediterranean Sea.Scientific reports · 2021Article
- Diversity and distribution of marine heterotrophic bacteria from a large culture collection.BMC microbiology · 2020Article
- The Link Between the Ecology of the Prokaryotic Rare Biosphere and Its Biotechnological Potential.Frontiers in microbiology · 2020Review
- Article
- Age-Related Differences in the Gastrointestinal Microbiota of Chinstrap Penguins (Pygoscelis antarctica).PloS one · 2016Article
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Authors and funding
4 authors.
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Abstract
High-throughput sequencing (HTS) techniques have suggested the existence of a wealth of species with very low relative abundance: the rare biosphere. We attempted to exhaustively map this rare biosphere in two water samples by performing an exceptionally deep pyrosequencing analysis (~500,000 final reads per sample). Species data were derived by a 97% identity criterion and various parametric distributions were fitted to the observed counts. Using the best-fitting Sichel distribution we estimate a total species richness of 1,568-1,669 (95% Credible Interval) and 5,027-5,196 for surface and deep water samples respectively, implying that 84-89% of the total richness in those two samples was sequenced, and we predict that a quadrupling of the present sequencing effort would suffice to observe 90% of the total richness in both samples. Comparing the HTS results with a culturing approach we found that most of the cultured taxa were not obtained by HTS, despite the high sequencing effort. Culturing therefore remains a useful tool for uncovering marine bacterial diversity, in addition to its other uses for studying the ecology of marine bacteria.
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