Evidence map›Paper›PMID 27442190›Full record

ArticleCurrent eye research2017

Gene Expression Profile of Extracellular Matrix and Adhesion Molecules in the Human Normal Corneal Stroma.

Ying Liu, Hu Huang, Guoying Sun, Saeed Alwadani, Richard D Semba, Gerard A Lutty, Samuel Yiu, Deepak P Edward

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Ying Liua Wilmer Eye Institute , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Hu Huanga Wilmer Eye Institute , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Guoying Sund Joint Shantou International Eye Center , Shantou University and the Chinese University of Hong Kong , Shantou , China.
Saeed Alwadania Wilmer Eye Institute , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Richard D Sembaa Wilmer Eye Institute , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Gerard A Luttya Wilmer Eye Institute , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Samuel Yiua Wilmer Eye Institute , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Deepak P Edwarda Wilmer Eye Institute , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Johns Hopkins Medicine · USJohns Hopkins University · USChinese University of Hong Kong · CNHe Eye Hospital · CN

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Jiang Qian · 1985 to 2026
$22.1M
Systemic complement and age-related macular degeneration.R01EY024596 · NEI · JOHNS HOPKINS UNIVERSITY · PI SEMBA, RICHARD D · 2014 to 2016
$1.6M
NEI NIH HHS P30 EY001765NEI NIH HHS R01 EY024596
6 · The paper itself

Abstract

purposeThere is limited information on region-specific gene expression in the human corneal stroma. In this study, we aimed to investigate the expression profile of the extracellular matrix and adhesion molecules in the normal corneal stroma using laser capture microdissection (LCM) and molecular techniques.

methodsFrozen sections of human cornea without ocular disease were used to isolate the central and peripheral corneal stromal keratocytes by LCM. RNA was extracted from LCM-captured tissues and the RT

resultsThe gene expression profiling demonstrated that 35 out of the 84 extracellular matrix and adhesion molecules represented in the array were expressed in stromal keratocytes. Among them, 24 genes were not previously described in the corneal stroma. Two genes were found more abundantly expressed in the central stroma than in the periphery: TGFBI, COL6A2 (p < 0.05). ADAMTS13 was detected only in the central stroma. Proteomics and WB analysis confirmed the expression of 10 genes. Functional analysis revealed that most identified genes were presented in a core cluster that had multiple and strong associations with other genes.

conclusionThis study identified genes not previously described in the corneal stroma, revealed regional differences in gene expression between central and peripheral stroma, and also detected some interesting candidate genes that may play important roles in corneal function. These observations serve as the foundation to further investigate the molecular and cellular mechanisms regulating the pathogenesis of regional corneal stromal disorders such as keratoconus.

Indexed as

AdultAgedBlotting, WesternCell Adhesion MoleculesCorneal KeratocytesCorneal StromaExtracellular Matrix ProteinsGene ExpressionGene Expression ProfilingHealthy VolunteersHumansMicroarray AnalysisMiddle AgedPolymerase Chain ReactionProteomicsTandem Mass SpectrometryCell Adhesion MoleculesExtracellular Matrix ProteinsAdhesion moleculescorneal stromaextracellular matrixgene expressionlaser capture microdissection

Identifiers

PMID27442190
PMCPMC6011830
OpenAlexW2486582433

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.