Evidence map›Paper›PMID 27442082›Full record

ArticleCurrent eye research2017

LRP-1 Pathway Targeted Inhibition of Vascular Abnormalities in the Retina of Diabetic Mice.

Ahamed Hossain, Lamiya Tauhid, Ian Davenport, Thomas Huckaba, Richard Graves, Tarun Mandal, Syed Muniruzzaman, Syed A Ahmed, Partha S Bhattacharjee

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Wnt Signaling in Inner Blood-Retinal Barrier Maintenance.International journal of molecular sciences · 2021
    Review
  5. Review
  6. Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Ahamed Hossaina Department of Biology , Xavier University of Louisiana , New Orleans , LA , USA.
Lamiya Tauhidb School of Science and Engineering , Tulane University , New Orleans , LA , USA.
Ian Davenporta Department of Biology , Xavier University of Louisiana , New Orleans , LA , USA.
Thomas Huckabaa Department of Biology , Xavier University of Louisiana , New Orleans , LA , USA.
Richard Gravesc Division of Basic Pharmaceutical Sciences, College of Pharmacy , Xavier University of Louisiana , New Orleans , LA , USA.
Tarun Mandalc Division of Basic Pharmaceutical Sciences, College of Pharmacy , Xavier University of Louisiana , New Orleans , LA , USA.
Syed Muniruzzamana Department of Biology , Xavier University of Louisiana , New Orleans , LA , USA.
Syed A Ahmedd Division of Business , Xavier University of Louisiana , New Orleans , LA , USA.
Partha S Bhattacharjeea Department of Biology , Xavier University of Louisiana , New Orleans , LA , USA.
Xavier University of Louisiana · USTulane University · US

Funding

Xavier's RCMI Cancer Research ProgramG12MD007595 · NIMHD · XAVIER UNIVERSITY OF LOUISIANA · PI D'AMOUR, GENE, WANG, GUANGDI · 2012 to 2018
$15.7M
NIMHD NIH HHS G12 MD007595
6 · The paper itself

Abstract

purposeThe cell surface LDL (low-density lipoprotein) receptor-related protein-1 (LRP-1) is important for lipid transport and several cell signaling processes. Human apolipoprotein E (apoE) is a ligand of LRP-1. We previously reported that a short peptide (apoEdp) mimicking the LRP-1 binding region of apoE prevents hyperglycemia-induced retinal endothelial cell dysfunction in vitro. The in-vivo outcome of apoE-based peptidomimetic inhibition of LRP-1 in the treatment of diabetic retinopathy is unknown.

methodsSix months after streptozotocin induction of diabetes, male C57Bl/6 mice were intravitreally inoculated with apoEdp in a controlled release formulation. On the 15th day post-apoEdp treatment, mouse retinas were harvested to examine (1) blood-retinal-barrier (BRB) permeability by Evans blue dye, inflammatory leukostasis by concanavalin staining of leukocytes and LRP-1 pathway-related protein expression by Western blot analysis and gelatin zymography.

resultsIntravitreal apoEdp treatment of diabetic mice significantly reduced Evans blue extravasation and the number of adherent leukocytes in the diabetic mouse retinas. ApoEdp treatment inhibited the expression of extracellular matrix (ECM) degrading proteases heparanase and MMP-2, and restores the BRB tight junction proteins occludin and ZO-1. ApoEdp treatment also inhibited Wnt/β-catenin-related expression of pro-inflammatory molecules ICAM-1, HIF-1α, and VEGF through negative regulation by LRP-1.

conclusionIntravitreal apoEdp treatment of diabetic mice resulted a significant decrease in retinal vascular abnormalities through downregulation of LRP-1-related ECM protein degradation and Wnt/β-catenin-related pro-angiogenic molecules.

Indexed as

Angiogenesis InhibitorsAnimalsApolipoproteins EBlood GlucoseBlood-Retinal BarrierBlotting, WesternCapillary PermeabilityDiabetes Mellitus, ExperimentalDiabetic RetinopathyExtracellular Matrix ProteinsIntravitreal InjectionsLeukostasisLow Density Lipoprotein Receptor-Related Protein-1MaleMiceMice, Inbred C57BLAngiogenesis InhibitorsApoEdpL-W peptide, humanApolipoproteins EBlood GlucoseExtracellular Matrix ProteinsLow Density Lipoprotein Receptor-Related Protein-1Lrp1 protein, mousePeptide FragmentsReceptors, LDLTumor Suppressor ProteinsBRBdiabetic retinopathyECMLRP-1Wnt/β-catenin

Identifiers

PMID27442082
PMCPMC5499693
OpenAlexW2504637985

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.