ArticlePloS one2016
Changes in the Cytoplasmic Composition of Amino Acids and Proteins Observed in Staphylococcus aureus during Growth under Variable Growth Conditions Representative of the Human Wound Site.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Comparative proteomics analysis of MRSA under different experimental conditions.Scientific reports · 2025Article
- Targeting the Metabolic Paradigms in Cancer and Diabetes.Biomedicines · 2024Review
- Cytoplasmic amino acid profiles of clinical and ATCC 29213 strains of Staphylococcus aureus harvested at different growth phases.Biomolecules & biomedicine · 2023Article
- Transposon sequencing identifies genes impactingInfection and immunity · 2023Article
- Significant Changes in Cytoplasmic Amino Acid Composition Occur in the Transition between Mid-Exponential and Stationary Phases of Growth ofMicroorganisms · 2023Article
- Changes in Amino Acid Metabolism ofMicroorganisms · 2022Article
- Skin-to-blood pH shift triggers metabolome and proteome global remodelling inFrontiers in microbiology · 2022Article
- The Inhibition Effect of Linezolid With Reyanning Mixture on MRSA and its Biofilm is More Significant than That of Linezolid Alone.Frontiers in pharmacology · 2021Article
- Analysis of Cytoplasmic and Secreted Proteins ofMicroorganisms · 2020Article
- Phenotypic and Genotypic Characterization with MALDI-TOF-MS Based Identification ofInternational journal of environmental research and public health · 2020Article
- Novel Cytoplasmic Bacteriocin Compounds Derived fromPathogens (Basel, Switzerland) · 2020Article
- Selected Metabolites Profiling ofFrontiers in microbiology · 2020Article
- Structural and Functional Dynamics ofMicroorganisms · 2019Article
- Amino acids and proteomic acclimation of Staphylococcus aureus when incubated in a defined minimal medium supplemented with 5% sodium chloride.MicrobiologyOpen · 2019Article
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
- Hydrogen Oxidation Influences Glycogen Accumulation in a Verrucomicrobial Methanotroph.Frontiers in microbiology · 2019Article
- The Uptake and Release of Amino Acids byFrontiers in microbiology · 2019Article
- Alterations in amino acid metabolism during growth byHeliyon · 2018Article
- Adaptive Metabolism in Staphylococci: Survival and Persistence in Environmental and Clinical Settings.Journal of pathogens · 2018Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus is an opportunistic pathogen responsible for a high proportion of nosocomial infections. This study was conducted to assess the bacterial responses in the cytoplasmic composition of amino acids and ribosomal proteins under various environmental conditions designed to mimic those on the human skin or within a wound site: pH6-8, temperature 35-37°C, and additional 0-5% NaCl. It was found that each set of environmental conditions elicited substantial adjustments in cytoplasmic levels of glutamic acid, aspartic acid, proline, alanine and glycine (P< 0.05). These alterations generated characteristic amino acid profiles assessed by principle component analysis (PCA). Substantial alterations in cytoplasmic amino acid and protein composition occurred during growth under conditions of higher salinity stress implemented via additional levels of NaCl in the growth medium. The cells responded to additional NaCl at pH 6 by reducing levels of ribosomal proteins, whereas at pH 8 there was an upregulation of ribosomal proteins compared with the reference control. The levels of two ribosomal proteins, L32 and S19, remained constant across all experimental conditions. The data supported the hypothesis that the bacterium was continually responding to the dynamic environment by modifying the proteome and optimising metabolic homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.