ArticlePloS one2016
Comparison of the Performances of Five Primer Sets for the Detection and Quantification of Plasmodium in Anopheline Vectors by Real-Time PCR.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Detection of Plasmodium in Anopheles spp (Diptera: Nematocera: Culicidae) using molecular biology techniques: a systematic review.Revista do Instituto de Medicina Tropical de Sao Paulo · 2025Pooled it
- Outdoor residual spraying for malaria vector-control in Kayin (Karen) state, Myanmar: A cluster randomized controlled trial.PloS one · 2022Trial
- Article
- Identification of Southeast Asian Anopheles mosquito species with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a cross-correlation approach.Parasites & vectors · 2025Article
- Utility of plasma anti-gSG6-P1 IgG levels in determining changes in Anopheles gambiae bite rates in a rural area of Cameroon.Scientific reports · 2024Article
- Identification of Southeast Asian Anopheles mosquito species using MALDI-TOF mass spectrometry.PloS one · 2024Article
- Key considerations, target product profiles, and research gaps in the application of infrared spectroscopy and artificial intelligence for malaria surveillance and diagnosis.Malaria journal · 2023Review
- Using qPCR to compare the detection ofFrontiers in parasitology · 2023Article
- Molecular screening reveals non-uniform malaria transmission in western Kenya and absence of Rickettsia africae and selected arboviruses in hospital patients.Malaria journal · 2022Article
- Outdoor malaria vector species profile in dryland ecosystems of Kenya.Scientific reports · 2022Article
- Article
- Impact of outdoor residual spraying on the biting rate of malaria vectors: A pilot study in four villages in Kayin state, Myanmar.PloS one · 2020Article
- Malaria surveillance from both ends: concurrent detection of Plasmodium falciparum in saliva and excreta harvested from Anopheles mosquitoes.Parasites & vectors · 2019Article
- Contribution of Asymptomatic Plasmodium Infections to the Transmission of Malaria in Kayin State, Myanmar.The Journal of infectious diseases · 2019Article
- A previously unreported potential malaria vector in a dry ecology of Kenya.Parasites & vectors · 2019Article
- Population genetics of Anopheles funestus, the African malaria vector, Kenya.Parasites & vectors · 2019Article
- Detection of diverseWellcome open research · 2019Article
- Insights into malaria transmission among Anopheles funestus mosquitoes, Kenya.Parasites & vectors · 2018Article
- Detection of malaria sporozoites expelled during mosquito sugar feeding.Scientific reports · 2018Article
- Entomological determinants of malaria transmission in Kayin state, Eastern Myanmar: A 24-month longitudinal study in four villages.Wellcome open research · 2018Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time polymerase chain reaction (qrtPCR) has made a significant improvement for the detection of Plasmodium in anopheline vectors. A wide variety of primers has been used in different assays, mostly adapted from molecular diagnosis of malaria in human. However, such an adaptation can impact the sensitivity of the PCR. Therefore we compared the sensitivity of five primer sets with different molecular targets on blood stages, sporozoites and oocysts standards of Plasmodium falciparum (Pf) and P. vivax (Pv). Dilution series of standard DNA were used to discriminate between methods at low concentrations of parasite and to generate standard curves suitable for the absolute quantification of Plasmodium sporozoites. Our results showed that the best primers to detect blood stages were not necessarily the best ones to detect sporozoites. Absolute detection threshold of our qrtPCR assay varied between 3.6 and 360 Pv sporozoites and between 6 and 600 Pf sporozoites per mosquito according to the primer set used in the reaction mix. In this paper, we discuss the general performance of each primer set and highlight the need to use efficient detection methods for transmission studies.
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