ArticlePloS one2016
Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06475365 (The Effect of Salt Water Gargling on Swallowing Dysfunction Following Multi-Level Anterior Cervical Discectomy and Fusion), which is not on this map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Effect of Salt Water Gargling on Swallowing Dysfunction Following Multi-Level Anterior Cervical Discectomy and Fusion (ACDF): A Randomized Control Trial
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Role of Biotics as a Therapeutic Strategy for Oral Mucositis - A Systematic Review.Probiotics and antimicrobial proteins · 2024Pooled it
- Anti-inflammatory effect of salt water and chlorhexidine 0.12% mouthrinse after periodontal surgery: a randomized prospective clinical study.Clinical oral investigations · 2021Trial
- Role and Relevance of Warm Saline Mouth Rinses in Contemporary Dental Practice: A Narrative Review.Cureus · 2026Review
- Evaluation of the Effects of Various Types of Mouthwash on Enamel and Cementum of Permanent Teeth: An In Vitro Study.TheScientificWorldJournal · 2026Article
- A qualitative study of Yorùbá culture and the utilisation of modern dental services for oral health.BMC health services research · 2025Article
- Practice of self-medication to manage oral health issues in a community setting of Nepal.BMC oral health · 2025Article
- Oral Opportunistic Infection Induced by Stress and Silent Type 2 Diabetes Mellitus in Young Adult Patient: A Case Report.International medical case reports journal · 2025Article
- Mouthwashes: Alternatives and Future Directions.International dental journal · 2023Review
- Article
- Management of Chronic Ulcer Mimicking Oral Squamous Cell Carcinoma in Down Syndrome Child with Motoric Disorders.International medical case reports journal · 2022Article
- Is saltwater mouth rinse as effective as chlorhexidine following periodontal surgery?Evidence-based dentistry · 2021Article
- An exploration of the oral health beliefs and behaviors of people living with HIV in Mangalore, India: a qualitative study.BMC oral health · 2021Article
- Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior.International journal of dentistry · 2020Article
- Chemical Composition of East Asian Invasive Knotweeds, their Cytotoxicity and Antimicrobial Efficacy Against Cariogenic Pathogens: An In-Vitro Study.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells.Marine drugs · 2019Article
- Comparative Evaluation of Antiplaque Efficacy between Essential Oils with Alcohol-Based and Chlorhexidine with Nonalcohol-Based Mouthrinses.Journal of International Society of Preventive & Community Dentistry · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rinsing the mouth with sodium chloride (NaCl) solution is believed to promote healthy gum and improve oral ulcer healing. Scientific evidence to support this assumption is, however, lacking. This study aims to investigate the effect and clarify underlying mechanisms of short-term rinsing with NaCl on human gingival fibroblast (hGFs) wound healing. Isolated primary hGFs and human normal oral keratinocytes (hNOKs) were rinsed with 0-7.2% NaCl for 2 min, 3 times a day. Scratch-tests, trans-well migration assays and MTT activity were performed. mRNA expression was assessed of type-I collagen, fibronectin and FAK. Changes in FAK and F-actin were detected by immunofluorescence. KCl, NaH2PO4, KH2PO4 were used to clarify the molecules involved. Rinsing with 0.9-1.8% NaCl significantly promoted hGFs cell migration but not proliferation. However, it had no effect on hNOKs. Rinsing with 1.8% NaCl significantly up-regulated the expression of type-I collagen and fibronectin. FAK and F-actin, molecules responsible for cytoskeleton re-organization and cell migration, were also up-regulated. Cl- seemed to be essential since rinsing with KCl resulted in a similar effect as noted with NaCl. In conclusion, short-term rinsing with NaCl promoted hGFs migration, and increased the expression of extracellular matrix as well as cytoskeletal proteins. These data strongly support the long held belief in the benefits of using NaCl mouth-rinse.
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Registered trials
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