ArticleHeliyon2016
Size effect of engineered islets prepared using microfabricated wells on islet cell function and arrangement.
Article in Heliyon, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Subaqueous acoustic pressure system based one day heterotypic pseudo-islet spheroid formation with adipose derived stem cells for graft survival-related function enhancement.Bioactive materials · 2025Article
- Scaffold-free endocrine tissue engineering: role of islet organization and implications in type 1 diabetes.BMC endocrine disorders · 2025Review
- Designing Porosity-Tailored Hydrogel Sponges with Controlled Cell Positioning Using Dispersible, Autofragmented Sacrificial Microfibers.ACS omega · 2025Article
- Constitutive Pleiotrophin Deletion Results in a Phenotype with an Altered Pancreatic Morphology and Function in Old Mice.International journal of molecular sciences · 2024Article
- Extracellular electrophysiology on clonal human β-cell spheroids.Frontiers in endocrinology · 2024Article
- Microwell culture platform maintains viability and mass of human pancreatic islets.Frontiers in endocrinology · 2022Article
- Formation of Re-Aggregated Neonatal Porcine Islet Clusters ImprovesTransplant international : official journal of the European Society for Organ Transplantation · 2022Article
- Making human pancreatic islet organoids: Progresses on the cell origins, biomaterials and three-dimensional technologies.Theranostics · 2022Review
- Process parameter development for the scaled generation of stem cell-derived pancreatic endocrine cells.Stem cells translational medicine · 2021Article
- A 3D culture platform enables development of zinc-binding prodrugs for targeted proliferation of β cells.Science advances · 2020Article
- Rapid fabrication of sieved microwells and cross-flow microparticle trapping.Scientific reports · 2020Article
- Sacrificial Alginate-Assisted Microfluidic Engineering of Cell-Supportive Protein Microfibers for Hydrogel-Based Cell Encapsulation.ACS omega · 2020Article
- Controlled Heterotypic Pseudo-Islet Assembly of Human β-Cells and Human Umbilical Vein Endothelial Cells Using Magnetic Levitation.Tissue engineering. Part A · 2020Article
- Micro Three-Dimensional Neuronal Cultures Generate Developing Cortex-Like Activity Patterns.Frontiers in neuroscience · 2020Article
- Automation, Monitoring, and Standardization of Cell Product Manufacturing.Frontiers in bioengineering and biotechnology · 2020Review
- Abnormal regulation of glucagon secretion by human islet alpha cells in the absence of beta cells.EBioMedicine · 2019Article
- Translational assessment of a genetic engineering methodology to improve islet function for transplantation.EBioMedicine · 2019Article
- Preliminary Studies of the Impact of CXCL12 on the Foreign Body Reaction to Pancreatic Islets Microencapsulated in Alginate in Nonhuman Primates.Transplantation direct · 2019Article
- The liver surface as a favorable site for islet cell sheet transplantation in type 1 diabetes model mice.Regenerative therapy · 2018Article
- Endothelial and beta cell composite aggregates for improved function of a bioartificial pancreas encapsulation device.The International journal of artificial organs · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pancreatic islets are heterogeneous clusters mainly composed of α and β cells, and these clusters range in diameter from 50 to several hundred micrometers. Native small islets are known to have a higher insulin secretion ability in vitro and to provide better transplantation outcomes when compared with large islets. In this study, we prepared microengineered pseudo-islets from dispersed rat islet cells using precisely-fabricated agarose gel-based microwells with different diameters (100, 300, or 500 μm) to investigate the function and survival of islet cell aggregates with well-controlled sizes. We observed that dead cells were rarely present in the small pseudo-islets with an average diameter of ∼60 μm prepared using 100 μm microwells. In contrast, we observed more dead cells in the larger pseudo-islets prepared using 300 and 500 μm microwells. The relative amount of hypoxic cells was significantly low in the small pseudo-islets whereas a hypoxic condition was present in the core region of the larger pseudo-islets. In addition, we found that the small-sized pseudo-islets reconstituted the in vivo-tissue like arrangement of the α and β cells, and restored the high insulin secretory capacity in response to high glucose. These results clearly suggest that precise size control of pseudo-islets is essential for maintaining islet cell function and survival in vitro. The small-sized pseudo-islets may be advantageous for providing a better therapeutic approach for treating type 1 diabetes mellitus via islet reorganization and transplantation.
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Registered trials
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