ArticleBiotechnology journal2017
Label-free live cell imaging by Confocal Raman Microscopy identifies CHO host and producer cell lines.
Article in Biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 30 citations in OpenAlex.
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- Review
- Raman spectrum spectral imaging revealing the molecular mechanism of Berberine-induced Jurkat cell apoptosis and the receptor-mediated Berberine delivery system.Biomedical optics express · 2019Article
- Raman micro-spectroscopy for accurate identification of primary human bronchial epithelial cells.Scientific reports · 2018Article
- Following laser induced changes of plant phenylpropanoids by Raman microscopy.Scientific reports · 2018Article
- Gold Nanoparticles in Single-Cell Analysis for Surface Enhanced Raman Scattering.Molecules (Basel, Switzerland) · 2016Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
As a possible viable and non-invasive method to identify high producing cells, Confocal Raman Microscopy was shown to be able to differentiate CHO host cell lines and derivative production clones. Cluster analysis of spectra and their derivatives was able to differentiate between different producer cell lines and a host, and also distinguished between an intracellular region of high lipid and protein content that in structure resembles the Endoplasmic Reticulum. This ability to identify the ER may be a major contributor to the identification of high producers. PCA enabled the discrimination even of host cell lines and their subclones with inherently higher production capacity. The method is thus a promising option that may contribute to early, non-invasive identification of high potential candidates during cell line development and possibly could also be used for proof of identity of established production clones.
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Registered trials
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