ArticleScientific reports2016
Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
- Mutations of the Electron Transport Chain Affect Lifespan and ROS Levels inAntioxidants (Basel, Switzerland) · 2025Review
- Chromophore-Assisted Light Inactivation for Protein Degradation and Its Application in Biomedicine.Bioengineering (Basel, Switzerland) · 2024Review
- UNC-49 is a redox-sensitive GABAScience advances · 2023Article
- Genetically encoded photocatalytic protein labeling enables spatially-resolved profiling of intracellular proteome.Nature communications · 2023Article
- Mitochondrial succinate dehydrogenase function is essential for sperm motility and male fertility.iScience · 2022Article
- A reversible mitochondrial complex I thiol switch mediates hypoxic avoidance behavior in C. elegans.Nature communications · 2022Article
- A Photosensitized Singlet Oxygen (Molecules (Basel, Switzerland) · 2022Review
- Model systems in SDHx-related pheochromocytoma/paraganglioma.Cancer metastasis reviews · 2021Review
- Optical manipulation of molecular function by chromophore-assisted light inactivation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2021Review
- Regulation of Ion Channel Function by Gas Molecules.Advances in experimental medicine and biology · 2021Review
- Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems.Cell chemical biology · 2020Article
- Mitochondrial Reactive Oxygen Species Generated at the Complex-II Matrix or Intermembrane Space Microdomain Have Distinct Effects on Redox Signaling and Stress Sensitivity inAntioxidants & redox signaling · 2019Article
- Light-induced oxidant production by fluorescent proteins.Free radical biology & medicine · 2018Review
- Blue-Light Receptors for Optogenetics.Chemical reviews · 2018Review
- Singlet oxygen modification abolishes voltage-dependent inactivation of the sea urchin spHCN channel.The Journal of general physiology · 2018Article
- Green-Light-Activated Photoreaction via Genetic Hybridization of Far-Red Fluorescent Protein and Silk.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation.BMC biology · 2018Article
- Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium.Free radical biology & medicine · 2018Article
- Exercise and Mitochondrial Dynamics: Keeping in Shape with ROS and AMPK.Antioxidants (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory function. Chromophore-Assisted Light Inactivation (CALI) using genetically-encoded photosensitizers provides an opportunity to determine how individual respiratory chain components contribute to physiology following acute lf. As proof-of-concept, we expressed the 'singlet oxygen generator' miniSOG as a fusion with the SDHC subunit of respiratory complex II, encoded by mev-1 in C. elegans, using Mos1-mediated Single Copy Insertion. The resulting mev-1::miniSOG transgene complemented mev-1 mutant phenotypes in kn1 missense and tm1081(lf) deletion mutants. Complex II activity was inactivated by blue light in mitochondria from strains expressing active miniSOG fusions, but not those from inactive fusions. Moreover, light-inducible phenotypes in vivo demonstrated that complex II activity is important under conditions of high energy demand, and that specific cell types are uniquely susceptible to loss of complex II. In conclusion, miniSOG-mediated CALI is a novel genetic platform for acute inactivation of respiratory chain components. Spatio-temporally controlled ROS generation will expand our understanding of how the respiratory chain and mitochondrial ROS influence whole organism physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.