ArticleJournal of immunology (Baltimore, Md. : 1950)2016
A Simple, Versatile Antibody-Based Barcoding Method for Flow Cytometry.
Article in Journal of immunology (Baltimore, Md. : 1950), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Single-cell omics technologies - Fundamentals on how to create single-cell looking glasses for reproductive health.American journal of obstetrics and gynecology · 2025Review
- A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen-specific T cells.Frontiers in cellular and infection microbiology · 2023Article
- Engagement of TRAIL triggers degranulation and IFNγ production in human natural killer cells.EMBO reports · 2022Article
- DebarcodeR increases fluorescent cell barcoding capacity and accuracy.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Flow-Cytometric Analysis and Purification of Airway Epithelial-Cell Subsets.American journal of respiratory cell and molecular biology · 2021Article
- High-Resolution Multicolor Imaging of Mitochondria in Lymphocytes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- High-Fidelity Single Molecule Quantification in a Flow Cytometer Using Multiparametric Optical Analysis.ACS nano · 2020Article
- High throughput pSTAT signaling profiling by fluorescent cell barcoding and computational analysis.Journal of immunological methods · 2020Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Regulatory T cells mediate specific suppression by depleting peptide-MHC class II from dendritic cells.Nature immunology · 2019Article
- Costimulatory Molecules and Immune Checkpoints Are Differentially Expressed on Different Subsets of Dendritic Cells.Frontiers in immunology · 2019Article
- The DifferentiationFrontiers in immunology · 2019Article
- Increased Mitochondrial Biogenesis and Reactive Oxygen Species Production Accompany Prolonged CD4Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Intrinsic properties of human germinal center B cells set antigen affinity thresholds.Science immunology · 2018Article
- Second signals rescue B cells from activation-induced mitochondrial dysfunction and death.Nature immunology · 2018Article
- Toll-like receptor 9 antagonizes antibody affinity maturation.Nature immunology · 2018Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies.European journal of immunology · 2017Article
- B Cells Produce Type 1 IFNs in Response to the TLR9 Agonist CpG-A Conjugated to Cationic Lipids.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Ex-vivo iTreg differentiation revisited: Convenient alternatives to existing strategies.Journal of immunological methods · 2017Article
- An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Barcoding of biological samples is a commonly used strategy to mark or identify individuals within a complex mixture. However, cell barcoding has not yet found wide use in flow cytometry that would benefit greatly from the ability to analyze pooled experimental samples simultaneously. This is due, in part, to technical and practical limitations of current fluorescent dye-based methods. In this study, we describe a simple, versatile barcoding strategy that relies on combinations of a single Ab conjugated to different fluorochromes and thus in principle can be integrated into any flow cytometry application. To demonstrate the efficacy of the approach, we describe the results of a variety of experiments using live cells as well as fixed and permeabilized cells. The results of these studies show that Ab-based barcoding provides a simple, practical method for identifying cells from individual samples pooled for analysis by flow cytometry that has broad applications in immunological research.
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Registered trials
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