ArticlePloS one2016
Fast Filtration of Bacterial or Mammalian Suspension Cell Cultures for Optimal Metabolomics Results.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Glutamine from plasma-like medium fuels nitrogen metabolism and influences proliferation in Leptospira interrogans.PLoS pathogens · 2026Article
- A Robust Isotope Ratio LC-MS/MS Workflow for High-Throughput Metabolic Profiling of Bacteria.Analytical chemistry · 2026Article
- Metabolites from plasma-like medium fuel nitrogen metabolism and influence proliferation inbioRxiv : the preprint server for biology · 2026Article
- Critical assessment of quenching and extraction/sample preparation methods for microorganisms in metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2025Review
- Unravelling lipid heterogeneity: Advances in single-cell lipidomics in cellular metabolism and disease.BBA advances · 2025Review
- Co-Culture of Gut Bacteria and Metabolite Extraction Using Fast Vacuum Filtration and Centrifugation.Methods and protocols · 2024Article
- Article
- Characterization and Amelioration of Filtration Difficulties Encountered in Metabolomic Studies of Clostridium thermocellum at Elevated Sugar Concentrations.Applied and environmental microbiology · 2023Article
- Optimized Fast Filtration-Based Sampling and Extraction Enables Precise and Absolute Quantification of theMetabolites · 2023Article
- Transcriptome and Metabolome Analysis Reveals the Importance of Amino-Acid Metabolism inInsects · 2022Article
- Clarification of large-volume bacterial cultures using a centrifuge-free protocol.Journal of applied microbiology · 2022Article
- Bacterial Metabolomics: Sample Preparation Methods.Biochemistry research international · 2022Review
- Metabolic profiling of attached and detached metformin and 2-deoxy-D-glucose treated breast cancer cells reveals adaptive changes in metabolome of detached cells.Scientific reports · 2021Article
- An Extensive Metabolomics Workflow to Discover Cardiotoxin-Induced Molecular Perturbations in Microtissues.Metabolites · 2021Article
- Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli.Scientific reports · 2021Article
- Past, Present, and Future of Affinity-based Cell Separation Technologies.Acta biomaterialia · 2020Review
- Mitochondrial aconitase is a key regulator of energy production for growth and protein expression in Chinese hamster ovary cells.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Prediction of liver toxicity and mode of action using metabolomics in vitro in HepG2 cells.Archives of toxicology · 2018Article
- Metabolite Profiling Reveals the Glutathione Biosynthetic Pathway as a Therapeutic Target in Triple-Negative Breast Cancer.Molecular cancer therapeutics · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The metabolome offers real time detection of the adaptive, multi-parametric response of the organisms to environmental changes, pathophysiological stimuli or genetic modifications and thus rationalizes the optimization of cell cultures in bioprocessing. In bioprocessing the measurement of physiological intracellular metabolite levels is imperative for successful applications. However, a sampling method applicable to all cell types with little to no validation effort which simultaneously offers high recovery rates, high metabolite coverage and sufficient removal of extracellular contaminations is still missing. Here, quenching, centrifugation and fast filtration were compared and fast filtration in combination with a stabilizing washing solution was identified as the most promising sampling method. Different influencing factors such as filter type, vacuum pressure, washing solutions were comprehensively tested. The improved fast filtration method (MxP® FastQuench) followed by routine lipid/polar extraction delivers a broad metabolite coverage and recovery reflecting well physiological intracellular metabolite levels for different cell types, such as bacteria (Escherichia coli) as well as mammalian cells chinese hamster ovary (CHO) and mouse myeloma cells (NS0).The proposed MxP® FastQuench allows sampling, i.e. separation of cells from medium with washing and quenching, in less than 30 seconds and is robustly designed to be applicable to all cell types. The washing solution contains the carbon source respectively the 13C-labeled carbon source to avoid nutritional stress during sampling. This method is also compatible with automation which would further reduce sampling times and the variability of metabolite profiling data.
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