ArticleTechnology2016
Dynamic deformability of sickle red blood cells in microphysiological flow.
Article in Technology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 71 citations in OpenAlex.
- Sickle cell visualization in vivo in humans: microvascular occlusion formation and hemorheological indices.Blood advances · 2026Article
- An AI-enabled tool for quantifying overlapping red blood cell sickling dynamics in microfluidic assays.Lab on a chip · 2026Article
- Pathophysiological Associations and Measurement Techniques of Red Blood Cell Deformability.Biosensors · 2025Review
- Article
- Stratification of βAntioxidants (Basel, Switzerland) · 2023Article
- Rapid single-cell physical phenotyping of mechanically dissociated tissue biopsies.Nature biomedical engineering · 2023Article
- iCLOTS: open-source, artificial intelligence-enabled software for analyses of blood cells in microfluidic and microscopy-based assays.Nature communications · 2023Article
- Advances in Microfluidics for Single Red Blood Cell Analysis.Biosensors · 2023Review
- Reconfigurable Vortex-like Paramagnetic Nanoparticle Swarm with Upstream Motility and High Body-length Ratio Velocity.Research (Washington, D.C.) · 2023Article
- Article
- Article
- Integrating deep learning with microfluidics for biophysical classification of sickle red blood cells adhered to laminin.PLoS computational biology · 2021Article
- Oxidative stress activates red cell adhesion to laminin in sickle cell disease.Haematologica · 2021Article
- Laser tweezers as a biophotonic tool to investigate the efficacy of living sickle red blood cells in response to optical deformation.Biophysical reviews · 2021Article
- Microfluidic electrical impedance assessment of red blood cell-mediated microvascular occlusion.Lab on a chip · 2021Article
- Concurrent Assessment of Deformability and Adhesiveness of Sickle Red Blood Cells by Measuring Perfusion of an Adhesive Artificial Microvascular Network.Frontiers in physiology · 2021Article
- A retrospective case study of successful translational research: Gazelle Hb variant point-of-care diagnostic device for sickle cell disease.Journal of clinical and translational science · 2021Article
- Whole blood viscosity and red blood cell adhesion: Potential biomarkers for targeted and curative therapies in sickle cell disease.American journal of hematology · 2020Article
- Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics.International journal of molecular sciences · 2020Review
- SC411 treatment can enhance survival in a mouse model of sickle cell disease.Prostaglandins, leukotrienes, and essential fatty acids · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
In sickle cell disease (SCD), hemoglobin molecules polymerize intracellularly and lead to a cascade of events resulting in decreased deformability and increased adhesion of red blood cells (RBCs). Decreased deformability and increased adhesion of sickle RBCs lead to blood vessel occlusion (vaso-occlusion) in SCD patients. Here, we present a microfluidic approach integrated with a cell dimensioning algorithm to analyze dynamic deformability of adhered RBC at the single-cell level in controlled microphysiological flow. We measured and compared dynamic deformability and adhesion of healthy hemoglobin A (HbA) and homozygous sickle hemoglobin (HbS) containing RBCs in blood samples obtained from 24 subjects. We introduce a new parameter to assess deformability of RBCs: the dynamic deformability index (DDI), which is defined as the time-dependent change of the cell's aspect ratio in response to fluid flow shear stress. Our results show that DDI of HbS-containing RBCs were significantly lower compared to that of HbA-containing RBCs. Moreover, we observed subpopulations of HbS containing RBCs in terms of their dynamic deformability characteristics: deformable and non-deformable RBCs. Then, we tested blood samples from SCD patients and analyzed RBC adhesion and deformability at physiological and above physiological flow shear stresses. We observed significantly greater number of adhered non-deformable sickle RBCs than deformable sickle RBCs at flow shear stresses well above the physiological range, suggesting an interplay between dynamic deformability and increased adhesion of RBCs in vaso-occlusive events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.