Evidence map›Paper›PMID 27437173›Full record

ArticleApplications in plant sciences2016

Low-coverage, whole-genome sequencing of Artocarpus camansi (Moraceae) for phylogenetic marker development and gene discovery.

Elliot M Gardner, Matthew G Johnson, Diane Ragone, Norman J Wickett, Nyree J C Zerega

Open access · goldAbstract read
In one paragraph

Article in Applications in plant sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 43 citations in OpenAlex.

  1. Article
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  4. Echoes of ancient introgression punctuate stable genomic lineages in the evolution of figs.Proceedings of the National Academy of Sciences of the United States of America · 2023
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  15. Practical considerations for plant phylogenomics.Applications in plant sciences · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Elliot M GardnerDepartment of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA; Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Hogan 2-144, Evanston, Illinois 60208 USA.
Matthew G JohnsonDepartment of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
Diane RagoneBreadfruit Institute, National Tropical Botanical Garden, Kalaheo, Hawaii 96741 USA.
Norman J WickettDepartment of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA; Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Hogan 2-144, Evanston, Illinois 60208 USA.
Nyree J C ZeregaDepartment of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA; Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Hogan 2-144, Evanston, Illinois 60208 USA.
Northwestern University · USChicago Botanic GardenNational Tropical Botanical Garden · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyWe used moderately low-coverage (17×) whole-genome sequencing of Artocarpus camansi (Moraceae) to develop genomic resources for Artocarpus and Moraceae. METHODS AND

resultsA de novo assembly of Illumina short reads (251,378,536 pairs, 2 × 100 bp) accounted for 93% of the predicted genome size. Predicted coding regions were used in a three-way orthology search with published genomes of Morus notabilis and Cannabis sativa. Phylogenetic markers for Moraceae were developed from 333 inferred single-copy exons. Ninety-eight putative MADS-box genes were identified. Analysis of all predicted coding regions resulted in preliminary annotation of 49,089 genes. An analysis of synonymous substitutions for pairs of orthologs (Ks analysis) in M. notabilis and A. camansi strongly suggested a lineage-specific whole-genome duplication in Artocarpus.

conclusionsThis study substantially increases the genomic resources available for Artocarpus and Moraceae and demonstrates the value of low-coverage de novo assemblies for nonmodel organisms with moderately large genomes.

Indexed as

Artocarpus camansibreadfruitbreadnutgenomeMADS boxMoraceae

Identifiers

PMID27437173
PMCPMC4948901
OpenAlexW2471992226

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.